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CyFlow CD15 Pacific Orange

品番 AS196272
抗体名 Anti-Hu CD15 PAC OR,MEM-158
包装単位 100 tests
濃度 -
容量 0.4 ml
関連製品
(アイソタイプコントロール)
-
反応性|交差吸着 Human レーザー Violet
抗原 CD15, Lewis X 最大蛍光波長 551 nm
クローン MEM-158 最大励起波長 400 nm
ホスト Mouse 標識/Format Pacific Orange™
アイソタイプ IgM 研究分野 Immunophenotyping | Blood group antigens
クローナリティ monoclonal 用途

Flow cytometry

Anti-Hu CD15 PAC OR,MEM-158

特異性

The antibody MEM-158 recognizes CD15 antigen, a cell membrane molecule 3-fucosyl-N-acetyllactosamine (3-FAL) strongly expressed on granulocytes, monocytes, macrophages, mast cells; it is also present on Langerhans cells and some myeloid precursors cells.

抗原情報

CD15 (Le(x); Lewis x or SSEA-1; stage specific embryonic antigen-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumor cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin´s disease.

利用方法

The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Benharroch D, Dima E, Levy A, Ohana-Malka O, Ariad S, Prinsloo I, Mejirovsky E, Sacks M, Gopas J: Differential expression of sialyl and non‑sialyl‑CD15 antigens on Hodgkin‑Reed‑Sternberg cells: significance in Hodgkin's disease. Leuk·Lymphoma. 2000·Sep; 39(1‑2):185‑94. <·PMID:·10975398·>
• Hakomori S: Le(X) and related structures as adhesion molecules. Histochem·J. 1992·Nov; 24(11):771‑6. <·PMID:·1362193·>
• Li C, Wong P, Pan T, Xiao F, Yin S, Chang B, Kang SC, Ironside J, Sy MS: Normal cellular prion protein is a ligand of selectins: binding requires Le(X) but is inhibited by sLe(X). Biochem·J. 2007·Sep·1; 406(2):333‑41. <·PMID:·17497959·>
• Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland·Publishing·Inc,·New·York. 1997; 1‑1342. <·NLM·ID:·9712219·>