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CyFlow CD5 PerCP-Cy5.5

品番 AS179656
抗体名 Anti-Hu CD5 PERCP-CY5.5,L17F12
包装単位 100 tests
濃度 -
容量 0.4 ml
関連製品
(アイソタイプコントロール)
-
反応性|交差吸着 Human レーザー Blue, Green
抗原 CD5, Leu-1 最大蛍光波長 695 nm
クローン L17F12 最大励起波長 482 nm
ホスト Mouse 標識/Format PerCP-Cy5.5
アイソタイプ IgG2a 研究分野 Immunophenotyping
クローナリティ monoclonal 用途

Flow cytometry

Anti-Hu CD5 PERCP-CY5.5,L17F12

特異性

The mouse monoclonal antibody L17F12 recognizes CD5 antigen, a 67kDa single-chain transmembrane glycoprotein expressed on mature T lymphocytes, most of thymocytes and B lymphocytes subset (B-1a lymphocytes).

抗原情報

CD5 (T1) is a human cell surface T-lymphocyte single-chain transmembrane glycoprotein. CD5 is expressed on all mature T-lymphocytes, most of thymocytes, subset of B-lymphocytes and on many T-cell leukemias and lymphomas. It is a type I membrane glycoprotein whose extracellular region contains three scavenger receptor cysteine-rich (SRCR) domains. The CD5 is a signal transducing molecule whose cytoplasmic tail is devoid of any intrinsic catalytic activity. CD5 modulates signaling through the antigen-specific receptor complex (TCR and BCR). CD5 crosslinking induces extracellular Ca++ mobilization, tyrosine phosphorylation of intracellular proteins and DAG production. Preliminary evidence shows protein associations with ZAP-70, p56lck, p59fyn, PC-PLC, etc. CD5 may serve as a dual receptor, giving either stimulatory or inhibitory signals depending both on the cell type and development stage. In thymocytes and B1a cells seems to provide inhibitory signals, in peripheral mature T lymhocytes it acts as a costimulatory signal receptor. CD5 is the phenotypic marker of a B cell subpopulation involved in the production of autoreactive antibodies.Disease relevance: CD5 is a phenotypic marker for some B cell lymphoproliferative disorders (B-CLL, Hairy cell leukemia, etc.). The CD5+ popuation is expanded in some autoimmune disorders (Rheumatoid Arthritis, etc.). Herpes virus infections induce loss of CD5 expression in the expanded CD8+ human T cells.

利用方法

The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Dunphy CH, Tang W: The value of CD64 expression in distinguishing acute myeloid leukemia with monocytic differentiation from other subtypes of acute myeloid leukemia: a flow cytometric analysis of 64 cases. Arch·Pathol·Lab·Med. 2007·May; 131(5):748‑54. <·PMID:·17488160·>
• Engleman EG, Warnke R, Fox RI, Dilley J, Benike CJ, Levy R: Studies of a human T lymphocyte antigen recognized by a monoclonal antibody. Proc·Natl·Acad·Sci·USA. 1981·Mar; 78(3):1791‑5. <·PMID:·7015346·>
• Gong JZ, Lagoo AS, Peters D, Horvatinovich J, Benz P, Buckley PJ: Value of CD23 determination by flow cytometry in differentiating mantle cell lymphoma from chronic lymphocytic leukemia/small lymphocytic lymphoma. Am·J·Clin·Pathol. 2001·Dec; 116(6):893‑7. <·PMID:·11764079·>
• McAlister MS, Davis B, Pfuhl M, Driscoll PC: NMR analysis of the N‑terminal SRCR domain of human CD5: engineering of a glycoprotein for superior characteristics in NMR experiments. Protein·Eng. 1998·Oct; 11(10):847‑53. <·PMID:·9862202·>
• Shuster JJ, Falletta JM, Pullen DJ, Crist WM, Humphrey GB, Dowell BL, Wharam MD, Borowitz M: Prognostic factors in childhood T‑cell acute lymphoblastic leukemia: a Pediatric Oncology Group study. Blood. 1990·Jan·1; 75(1):166‑73. <·PMID:·1688495·>

Available Safety Data Sheets

CyFlow™ monoclonal antibodies SDS CA EN

Available Technical Data Sheets

CyFlow™ CD5 PerCP-Cy5.5 (L17F12) AS179656 TDS (US)