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CyFlow IgG2b Purified

品番 AS131472
抗体名 Mouse IGG2B PURI,MPC-11
包装単位 0.1 mg
濃度 1 mg/ml
容量 0.1 ml
関連製品
(アイソタイプコントロール)
-
反応性|交差吸着 レーザー
抗原 IgG2b Isotype Control 最大蛍光波長 -
クローン MPC-11 最大励起波長 -
ホスト Mouse 標識/Format Purified
アイソタイプ IgG2b 研究分野 Isotype controls
クローナリティ monoclonal 用途

Flow cytometry (Isotype control)

Mouse IGG2B PURI,MPC-11

特異性

This mouse IgG2b κ monoclonal antibody (clone MPC-11) reacts with an epitope irrelevant for a variety of resting, activated, live, and fixed human, mouse, and rat tissues.

抗原情報

The specificity of staining by monoclonal antibodies to target antigens should be verified by establishing the amount of non-specific antibody binding. Especially at higher concentration (more than 15 μ/ml) the antibody staining usually has consignable background. To this end a non-reactive immunoglobulin of the same isotype is included as a negative control for each specific monoclonal antibody used in a particular immunoassay. The monoclonal antibody MPC-11 was generated against an epitope irrelevant for human, mouse, and rat material, and can thus be used for evaluation of the background staining that is caused by general nonspecific interactions between an mouse IgG2b molecule and the respective sample under the particular conditions. This shall help the customer to set up the experimental conditions so that the nonspecific binding of any antibody is abolished.

利用方法

The reagent is designed as an isotype control for Flow Cytometry analysis. To establish the amount of non-specific antibody binding, match the concentration of the correct isotype to the recommended working concentration of the antigen-specific antibody. If the background signal of the isotype control is too high (usually when working antibody concentrations are above 10 µg/ml of incubation mixture), change the experimental conditions to reduce the background.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Dewals BG, Vanderplasschen A: Malignant catarrhal fever induced by Alcelaphine herpesvirus 1 is characterized by an expansion of activated CD3+CD8+CD4‑ T cells expressing a cytotoxic phenotype in both lymphoid and non‑lymphoid tissues.. Vet·Res. 2011·Aug·22; 42:95. <·PMID:·21859474·>
• Gupta D, Wang Q, Vinson C, Dziarski R: Bacterial peptidoglycan induces CD14‑dependent activation of transcription factors CREB/ATF and AP‑1. J·Biol·Chem. 1999·May·14; 274(20):14012‑20. <·PMID:·10318814·>
• Im JS, Tapinos N, Chae GT, Illarionov PA, Besra GS, DeVries GH, Modlin RL, Sieling PA, Rambukkana A, Porcelli SA: Expression of CD1d molecules by human schwann cells and potential interactions with immunoregulatory invariant NK T cells. J·Immunol. 2006·Oct·15; 177(8):5226‑35. <·PMID:·17015708·>
• Liang XS, Zhou Y, Li CZ, Wan MB: Natural course of chronic hepatitis B is characterized by changing patterns of programmed death type‑1 of CD8‑positive T cells. World·J·Gastroenterol. 2010·Feb·7; 16(5):618‑24. <·PMID:·20128032·>
• Smed-Sörensen A, Moll M, Cheng TY, Loré K, Norlin AC, Perbeck L, Moody DB, Spetz AL, Sandberg JK: IgG regulates the CD1 expression profile and lipid antigen‑presenting function in human dendritic cells via FcgammaRIIa. Blood. 2008·May·15; 111(10):5037‑46. <·PMID:·18337560·>

Available Safety Data Sheets

CyFlow™ monoclonal antibodies SDS CA EN

Available Technical Data Sheets

CyFlow™ IgG2b Purified (MPC-11) AS131472 TDS (US)