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CyFlow CD3 Low Endotoxin

品番 AQ624194
抗体名 Anti-Hu CD3 LOW END,UCHT1
包装単位 0.1 mg
濃度 1 mg/ml
容量 0.1 ml
関連製品
(アイソタイプコントロール)
Mouse IgG1 Low Endotoxin (AK478344)
反応性|交差吸着 Human | Non-Human Primates レーザー
抗原 CD3, T3, Leu4 最大蛍光波長 -
クローン UCHT1 最大励起波長 -
ホスト Mouse 標識/Format Low Endotoxin
アイソタイプ IgG1 研究分野 Immunophenotyping
クローナリティ monoclonal 用途

Flow cytometry
Immunoprecipitation
Immunohistochemistry (frozen sections)
Functional assays

Anti-Hu CD3 LOW END,UCHT1

特異性

The mouse monoclonal antibody UCHT1 recognizes the CD3 antigen of the TCR/CD3 complex on mature human T cells. The UCHT1 antibody reacts with the ε chain of the CD3 complex. The monoclonal antibodies UCHT1 and SK7 recognize overlapping epitopes.

抗原情報

CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 γ, CD3 δ, CD3 ε and CD3 ζ. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

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• Arnett KL, Harrison SC, Wiley DC: Crystal structure of a human CD3‑epsilon/delta dimer in complex with a UCHT1 single‑chain antibody fragment. Proc·Natl·Acad·Sci·USA. 2004·Nov·16; 101(46):16268‑73. <·PMID:·15534202·>
• Barclay, Brown, et al.: The Leukocyte Antigen FactsBook; 2nd edition. Harcourt·Brace·&·Company,·London. 1997; <·No·PMID·>
• Demedts IK, Brusselle GG, Vermaelen KY, Pauwels RA: Identification and characterization of human pulmonary dendritic cells. Am·J·Respir·Cell·Mol·Biol. 2005·Mar; 32(3):177‑84. <·PMID:·15576669·>
• Fisch P, Malkovsky M, Braakman E, Sturm E, Bolhuis RL, Prieve A, Sosman JA, Lam VA, Sondel PM: Gamma/delta T cell clones and natural killer cell clones mediate distinctpatterns of non‑major histocompatibility complex‑restricted cytolysis. J·Exp·Med. 1990·May·1; 171(5):1567‑79. <·PMID:·2185329·>
• Garson JA, Beverley PC, Coakham HB, Harper EI: Monoclonal antibodies against human T lymphocytes label Purkinje neurones of many species. Nature. 1982·Jul·22; 298(5872):375‑7. <·PMID:·6178042·>
• Huang Y, Wange RL: T cell receptor signaling: beyond complex complexes. J·Biol·Chem. 2004·Jul·9; 279(28):28827‑3. <·PMID:·15084594·>
• Kuhns MS, Davis MM, Garcia KC: Deconstructing the form and function of the TCR/CD3 complex. Immunity. 2006·Feb; 24(2):133‑9. <·PMID:·16473826·>
• le Gouvello S, Manceau V, Sobel A: Serine 16 of stathmin as a cytosolic target for Ca2+/calmodulin‑dependent kinase II after CD2 triggering of human T lymphocytes. J·Immunol. 1998·Aug·1; 161(3):1113‑22. <·PMID:·9686569·>
• Lin CW, Liu TY, Chen SU, Wang KT, Medeiros LJ, Hsu SM: CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features. Blood. 2005·Nov·15; 106(10):3567‑74. <·PMID:·16046525·>
• Rieux-Laucat F, Hivroz C, Lim A, Mateo V, Pellier I, Selz F, Fischer A, Le Deist F: Inherited and somatic CD3zeta mutations in a patient with T‑cell deficiency. N·Engl·J·Med. 2006·May·4; 354(18):1913‑21. <·PMID:·16672702·>
• Siegers GM, Swamy M, Fernández-Malavé E, Minguet S, Rathmann S, Guardo AC, Pérez-Flores V, Regueiro JR, Alarcón B, Fisch P, Schamel WW: Different composition of the human and the mouse gammadelta T cell receptor explains different phenotypes of CD3gamma and CD3delta immunodeficiencies. J·Exp·Med. 2007·Oct·29; 204(11):2537‑44. <·PMID:·17923503·>
• Torres PS, Alcover A, Zapata DA, Arnaud J, Pacheco A, Martín-Fernández JM, Villasevil EM, Sanal O, Regueiro JR: TCR dynamics in human mature T lymphocytes lacking CD3 gamma. J·Immunol. 2003·Jun·15; 170(12):5947‑55. <·PMID:·12794121·>
• McMichael AJ, Beverley PCL, Cobbold S, et al (Eds): Leucocyte Typing III, White Cell Differentiation Antigens. Oxford·University·Press,·Oxford. 1987; 1‑1050. <·NLM·ID:·8913266·>
• Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland·Publishing·Inc,·New·York. 1997; 1‑1342. <·NLM·ID:·9712219·>

Available Safety Data Sheets

CyFlow™ monoclonal antibodies SDS CA EN

Available Technical Data Sheets

CyFlow™ CD3 Low Endotoxin (UCHT1) AQ624194 TDS (US)