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CyFlow CD222 Alexa Fluor 647

品番 AQ486061
抗体名 Anti-Hu CD222 AF 647,MEM-238
包装単位 100 tests
濃度 -
容量 0.4 ml
関連製品
(アイソタイプコントロール)
Mouse IgG1 Alexa Fluor™ 647 (AP602054)
反応性|交差吸着 Human | Non-Human Primates レーザー Red
抗原 CD222, IGF2R 最大蛍光波長 665 nm
クローン MEM-238 最大励起波長 650 nm
ホスト Mouse 標識/Format Alexa Fluor™ 647
アイソタイプ IgG1 研究分野 Immunophenotyping | Signaling
クローナリティ monoclonal 用途

Flow cytometry

Anti-Hu CD222 AF 647,MEM-238

特異性

The mouse monoclonal antibody MEM-238 recognizes an epitope between domains 2 and 5 of CD222 antigen, a ubiquitously expressed 250 kDa multifunctional type I transmembrane protein. The majority of CD222 is found in the late endosomal/prelysosomal compartment, 5-10% in the plasma membrane and the truncated (220 kDa) form of CD222 is present in human and bovine serum.

抗原情報

CD222 (CIMPR; cation-independent mannose 6-phosphate receptor or IGF2 receptor) is a ubiquitously expressed 250 kDa transmembrane protein. No more than 10% of CD222 is present on the cell surface where it serves as a multifunctional receptor. Intracellular (major) fraction of CD222 is involved in transport of newly synthesized lysosomal enzymes modified by mannose 6-phosphate from Golgi apparatus to lysosomes. The cell surface CD222 binds and internalizes exogeneous mannose 6-phosphate-containing ligands. Importantly, CD222 is crutial for internalization and degradation of insulin-like growth factor 2, thus controling cell growth. CD222 also complexes CD87 (urokinase-type plasminogen-activator receptor), plasminogen and latent TGF-β, last but not least CD222 serves as a receptor for heparanase and even for Listeria.

利用方法

The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Gasanov U, Koina C, Beagley KW, Aitken RJ, Hansbro PM: Identification of the insulin‑like growth factor II receptor as a novel receptor for binding and invasion by Listeria monocytogenes. Infect·Immun. 2006·Jan; 74(1):566‑77. <·PMID:·16369013·>
• Leksa V, Godar S, Cebecauer M, Hilgert I, Breuss J, Weidle UH, Horejsi V, Binder BR, Stockinger H: The N terminus of mannose 6‑phosphate/insulin‑like growth factor 2 receptor in regulation of fibrinolysis and cell migration. J·Biol·Chem. 2002·Oct·25; 277(43):40575‑82. <·PMID:·12189157·>
• Wood RJ, Hulett MD: Cell surface‑expressed cation‑independent mannose 6‑phosphate receptor (CD222 binds enzymatically active heparanase independently of mannose 6‑phosphate to promote extracellular matrix degradation. J·Biol·Chem. 2008·Feb·15; 283(7):4165‑76. <·PMID:·18073203·>
• Mason D, Simmons D, Buckley C, Schwartz-Albiez R, Hadam M, Saalmuller A, Clark E, Malavasi F, Morrissey JA (Eds): Leucocyte Typing VII. Oxford·University·Press. 2002; 1‑945. <·NLM·ID:·101177131·>