特異性

The mouse monoclonal antibody T21/8 recognizes the N-teminus of CD195 antigen, a 45 kDa G-protein coupled receptor 1 family protein expressed on resting T cells, monocytes, macrophages, and immature dendritic cells.

抗原情報

CD195 (CCR5, CKR-5) is a receptor for inflammatory CC-chemokines (characterized by a pair of adjacent cysteine residues), such as MIP-1 α, MIP-1 β, or RANTES. It is a G protein-associated seven-pass transmembrane protein expressed on resting T cells with memory/effector phenotype, monocytes, macrophages and immature dendritic cells. This chemokine receptor regulates the activation and directed migration of leukocytes. Importantly, along with CD4, CD195 functions as a major receptor for HIV. Their ligand is the viral glycoprotein gp120.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Hüttenrauch F, Pollok-Kopp B, Oppermann M: G protein‑coupled receptor kinases promote phosphorylation and beta‑arrestin‑mediated internalization of CCR5 homo‑ and hetero‑oligomers. J·Biol·Chem. 2005·Nov·11; 280(45):37503‑15. <·PMID:·16144840·>
• Mascalchi P, Lamort AS, Salomé L, Dumas F: Single Particle Tracking reveals two distinct environments for CD4 receptors at the surface of living T lymphocytes. Biochem·Biophys·Res·Commun. 2012·Jan·6; 417(1):409‑13. <·PMID:·22166195·>
• Monde K, Maeda Y, Tanaka Y, Harada S, Yusa K: Gp120 V3‑dependent impairment of R5 HIV‑1 infectivity due to virion‑incorporated CCR5. J·Biol·Chem. 2007·Dec·21; 282(51):36923‑32. <·PMID:·17971448·>
• Pollok-Kopp B, Schwarze K, Baradari VK, Oppermann M: Analysis of ligand‑stimulated CC chemokine receptor 5 (CCR5) phosphorylation in intact cells using phosphosite‑specific antibodies. J·Biol·Chem. 2003·Jan·24; 278(4):2190‑8. <·PMID:·12403770·>