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CyFlow CD80 PerCP

品番 AP655226
抗体名 Anti-Hu CD80 PERCP,MEM-233
包装単位 100 tests
濃度 -
容量 1 ml
関連製品
(アイソタイプコントロール)
Mouse IgG1 PerCP (AH500793)
反応性|交差吸着 Human レーザー Blue, Green
抗原 CD80, B7- B7-1, BB1 最大蛍光波長 678 nm
クローン MEM-233 最大励起波長 482 to 490 nm
ホスト Mouse 標識/Format PerCP
アイソタイプ IgG1 研究分野 Immunophenotyping
クローナリティ monoclonal 用途

Flow cytometry

Anti-Hu CD80 PERCP,MEM-233

特異性

The mouse monoclonal antibody MEM-233 recognizes CD80 antigen, a 60 kDa single chain type I glycoprotein of immunoglobulin supergene family, expressed on professional antigen-presenting cells, such as dendritic cells, macrophages or activated B lymphocytes.

抗原情報

CD80 (B7-1) and CD86 (B7-2) are ligands of T cell critical costimulatory molecule CD28 and of an inhibitory receptor CD152 (CTLA-4). Both B7 molecules are expressed on professional antigen-presenting cells and are essential for T cell activation, the both molecules can also substitute for each other in this process. The question what are the differences in CD80 and CD86 competency has not been fully elucidated yet; there are still conflicts in results about their respective roles in initiation or sustaining of the T cell immune response.

利用方法

The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Campioni D, Moretti S, Ferrari L, Punturieri M, Castoldi GL, Lanza F: Immunophenotypic heterogeneity of bone marrow‑derived mesenchymal stromal cells from patients with hematologic disorders: correlation with bone marrow microenvironment. Haematologica. 2006·Mar; 91(3):364‑8. <·PMID:·16531259·>
• Eri R, Kodumudi KN, Summerlin DJ, Srinivasan M: Suppression of colon inflammation by CD80 blockade: evaluation of two murine models of inflammatory bowel disease. Inflamm·Bowel·Dis. 2008·Jan·9; <·PMID:·21061388·>
• Hovden AO, Karlsen M, Jonsson R, Aarstad HJ, Appel S: Maturation of monocyte derived dendritic cells with OK432 boosts IL‑12p70 secretion and conveys strong T‑cell responses. BMC·Immunol. 2011·Jan·5; 12:2. <·PMID:·21208424·>
• Tan PH, Beutelspacher SC, Wang YH, McClure MO, Ritter MA, Lombardi G, George AJ: Immunolipoplexes: an efficient, nonviral alternative for transfection of human dendritic cells with potential for clinical vaccination. Mol·Ther. 2005·May; 11(5):790‑800. <·PMID:·15851017·>
• Kolar GR, Mehta D, Pelayo R, Capra JD: A novel human B cell subpopulation representing the initial germinal center population to express AID. Blood. 2007·Mar·15; 109(6):2545‑52. <·PMID:·17132718·>
• Lee DJ, Sieling PA, Ochoa MT, Krutzik SR, Guo B, Hernandez M, Rea TH, Cheng G, Colonna M, Modlin RL: LILRA2 activation inhibits dendritic cell differentiation and antigen presentation to T cells. J·Immunol. 2007·Dec·15; 179(12):8128‑36. <·PMID:·18056355·>
• Silk KM, Leishman AJ, Nishimoto KP, Reddy A, Fairchild PJ: Rapamycin conditioning of dendritic cells differentiated from human ES cells promotes a tolerogenic phenotype. J·Biomed·Biotechnol. 2012; 2012:172420. <·PMID:·22505805·>
• Thomas IJ, Petrich de Marquesini LG, Ravanan R, Smith RM, Guerder S, Flavell RA, Wraith DC, Wen L, Wong FS: CD86 has sustained costimulatory effects on CD8 T cells. J·Immunol. 2007·Nov·1; 179(9):5936‑46. <·PMID:·17947667·>
• Vasilevko V, Ghochikyan A, Holterman MJ, Agadjanyan MG: CD80 (B7‑1 and CD86 (B7‑2 are functionally equivalent in the initiation and maintenance of CD4+ T‑cell proliferation after activation with suboptimal doses of PHA. DNA·Cell·Biol. 2002·Mar; 21(3):137‑49. <·PMID:·12015893·>
• Yadav D, Judkowski V, Flodstrom-Tullberg M, Sterling L, Redmond WL, Sherman L, Sarvetnick N: B7‑2 (CD86 controls the priming of autoreactive CD4 T cell response against pancreatic islets. J·Immunol. 2004·Sep·15; 173(6):3631‑9. <·PMID:·15356107·>
• Zhan H, Towler HM, Calder VL: The immunomodulatory role of human conjunctival epithelial cells. Invest·Ophthalmol·Vis·Sci. 2003·Sep; 44(9):3906‑10. <·PMID:·12939308·>

Available Safety Data Sheets

CyFlow™ monoclonal antibodies SDS CA EN

Available Technical Data Sheets

CyFlow™ CD80 PerCP (MEM-233) AP655226 TDS (US)