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CyFlow CD16 PE-Cy7

品番 AP596497
抗体名 Anti-Hu CD16 PE-CY7,3G8
包装単位 100 tests
濃度 -
容量 0.4 ml
関連製品
(アイソタイプコントロール)
Mouse IgG1 PE-Cy7 (CF148611)
反応性|交差吸着 Human | Non-Human Primates レーザー Blue, Green, Yellow
抗原 CD16, FcγRIIIA, CD16a 最大蛍光波長 785 nm
クローン 3G8 最大励起波長 496 nm, 565 nm
ホスト Mouse 標識/Format PE-Cy7
アイソタイプ IgG1 研究分野 Immunophenotyping
クローナリティ monoclonal 用途

Flow cytometry

Anti-Hu CD16 PE-CY7,3G8

特異性

The mouse monoclonal antibody 3G8 recognizes CD16 antigen, a low affinity receptor for aggregated IgG (FcγRIII antigen). CD16 exists in two different isoforms: CD16a (FcγRIIIA; 50-65 kDa; expressed on NK-cells, monocytes and macrophages) and CD16b (FcγRIIIB; 48 kDa; mainly expressed on neutrophils).

抗原情報

CD16 (FcγRIII) is a 50-65 kDa glycoprotein serving as a low affinity IgG receptor. Human FcγRIII is expressed in two forms - FcγRIIIA and FcγRIIIB. FcγRIIIA is a transmembrane protein of monocytes, macrophages, NK cells and a subset of T cells. It is associated with FcεRI-γ subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FcγRIIIA is associated, moreover, with FcεRI-β subunit. Besides IgG, FcγRIIIA can be triggered also by oligomeric IgE. FcγRIIIB is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a proadhesive phenotype.

利用方法

The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Burt BM, Plitas G, Zhao Z, Bamboat ZM, Nguyen HM, Dupont B, DeMatteo RP: The lytic potential of human liver NK cells is restricted by their limited expression of inhibitory killer Ig‑like receptors. J·Immunol. 2009·Aug·1; 183(3):1789‑96. <·PMID:·19587011·>
• Choi EI, Wang R, Peterson L, Letvin NL, Reimann KA: Use of an anti‑CD16 antibody for in vivo depletion of natural killer cells in rhesus macaques. Immunology. 2008·Jun; 124(2):215‑22. <·PMID:·18201184·>
• Congy-Jolivet N, Bolzec A, Ternant D, Ohresser M, Watier H, Thibault G: Fc gamma RIIIa expression is not increased on natural killer cells expressing the Fc gamma RIIIa‑158V allotype. Cancer·Res. 2008·Feb·15; 68(4):976‑80. <·PMID:·18281470·>
• Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J·Clin·Pathol. 1993·Apr; 46(4):334‑6. <·PMID:·7684403·>
• Komano Y, Nanki T, Hayashida K, Taniguchi K, Miyasaka N: Identification of a human peripheral blood monocyte subset that differentiates into osteoclasts. Arthritis·Res·Ther. 2006; 8(5):R152. <·PMID:·16987426·>
• Metes D, Ernst LK, Chambers WH, Sulica A, Herberman RB, Morel PA: Expression of functional CD32 molecules on human NK cells is determined by an allelic polymorphism of the FcgammaRIIC gene. Blood. 1998·Apr·1; 91(7):2369‑80. <·PMID:·9516136·>
• Wijngaarden S, van Roon JA, van de Winkel JG, Bijlsma JW, Lafeber FP: Down‑regulation of activating Fcgamma receptors on monocytes of patients with rheumatoid arthritis upon methotrexate treatment. Rheumatology·(Oxford). 2005·Jun; 44(6):729‑34. <·PMID:·15757966·>
• Zhu X, Hamann KJ, Muňoz NM, Rubio N, Mayer D, Herrnreiter A, Leff AR: Intracellular expression of Fc gamma RIII (CD16) and its mobilization by chemoattractants in human eosinophils. J·Immunol. 1998·Sep·1; 161(5):2574‑9. <·PMID:·9725258·>
• Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford·University·Press,·Oxford. 1989; 1‑1820. <·NLM·ID:·8914679·>

Available Safety Data Sheets

CyFlow™ monoclonal antibodies SDS CA EN

Available Technical Data Sheets

CyFlow™ CD16 PE-Cy7 (3G8) AP596497 TDS (US)