特異性

The mouse monoclonal antibody DBN-N-03 recognizes drebrin, an approximately 100-125 kDa regulator of actin cytoskeleton.

抗原情報

Drebrin is an actin-binding protein, which is expressed mainly in neurons and plays important role in their morphogenesis. The highest level of its expression is in developing brain. Both in neurons and non-neuronal cells drebrin acts as a key regulator of actin cytoskeleton remodelling, affecting especially intercellular junctions, such as dendritic spines of neurons or the immune synapses of T cells. Decrease of drebrin amount in the brain seems to be associated with Alzheimer's disease and Down syndrome, and in case of B-cell precursor acute lymphoblastic leukemia (BCP-ALL) lower drebrin expression correlates with higher risk of relapse.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Hayashi K, Ishikawa R, Kawai-Hirai R, Takagi T, Taketomi A, Shirao T: Domain analysis of the actin‑binding and actin‑remodeling activities of drebrin. Exp·Cell·Res. 1999·Dec·15; 253(2):673‑80. <·PMID:·10585290·>
• Hayashi K, Ishikawa R, Ye LH, He XL, Takata K, Kohama K, Shirao T: Modulatory role of drebrin on the cytoskeleton within dendritic spines in the rat cerebral cortex. J·Neurosci. 1996·Nov·15; 16(22):7161‑70. <·PMID:·8929425·>
• Ishikawa R, Hayashi K, Shirao T, Xue Y, Takagi T, Sasaki Y, Kohama K: Drebrin, a development‑associated brain protein from rat embryo, causes the dissociation of tropomyosin from actin filaments. J·Biol·Chem. 1994·Nov·25; 269(47):29928‑33. <·PMID:·7961990·>
• Pérez-Martínez M, Gordón-Alonso M, Cabrero JR, Barrero-Villar M, Rey M, Mittelbrunn M, Lamana A, Morlino G, Calabia C, Yamazaki H, Shirao T, Vázquez J, González-Amaro R, Veiga E, Sánchez-Madrid F: F‑actin‑binding protein drebrin regulates CXCR4 recruitment to the immune synapse. J·Cell·Sci. 2010·Apr·1; 123(7):1160‑70. <·PMID:·20215400·>
• Sasaki Y, Hayashi K, Shirao T, Ishikawa R, Kohama K: Inhibition by drebrin of the actin‑bundling activity of brain fascin, a protein localized in filopodia of growth cones. J·Neurochem. 1996·Mar; 66(3):980‑8. <·PMID:·8769857·>
• Vaskova M, Kovac M, Volna P, Angelisova P, Mejstrikova E, Zuna J, Brdicka T, Hrusak O: High expression of cytoskeletal protein drebrin in TEL/AML1pos B‑cell precursor acute lymphoblastic leukemia identified by a novel monoclonal antibody. Leuk·Res. 2011·Aug; 35(8):1111‑3. <·PMID:·21497902·>