CyFlow CD45 PerCP
| 品番 | AP194285 | ||
|---|---|---|---|
| 抗体名 | Anti-Ms CD45 PERCP,EM-05 | ||
| 包装単位 | 0.1 mg | ||
| 濃度 | 0.5 mg/ml | ||
| 容量 | 0.2 ml | ||
| 関連製品 (アイソタイプコントロール) |
- | ||
| 反応性|交差吸着 | Mouse | レーザー | Blue, Green |
| 抗原 | CD45, LCA, T200, B220 | 最大蛍光波長 | 678 nm |
| クローン | EM-05 | 最大励起波長 | 482 to 490 nm |
| ホスト | Rat | 標識/Format | PerCP |
| アイソタイプ | IgG | 研究分野 | Immunophenotyping | Phosphorylation | MHC |
| クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Ms CD45 PERCP,EM-05
特異性
The rat monoclonal antibody EM-05 recognizes mouse CD45 antigen, a single chain type I transmembrane protein expressed at high level on cells of hematopoietic origin, except erythrocytes and platelets.
抗原情報
CD45 (LCA; leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.
利用方法
The reagent is designed for Flow Cytometry analysis. Suggested working usage is 4 µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Byth KF, Conroy LA, Howlett S, Smith AJ, May J, Alexander DR, Holmes N: CD45‑null transgenic mice reveal a positive regulatory role for CD45 in earlythymocyte development, in the selection of CD4+CD8+ thymocytes, and B cell maturation. J·Exp·Med. 1996·Apr·1; 183(4):1707‑18. <·PMID:·8666928·>
• Dawes R, Petrova S, Liu Z, Wraith D, Beverley PC, Tchilian EZ: Combinations of CD45 isoforms are crucial for immune function and disease. J·Immunol. 2006·Mar·15; 176(6):3417‑25. <·PMID:·16517710·>
• Desharnais P, Dupéré-Minier G, Hamelin C, Devine P, Bernier J: Involvement of CD45 in DNA fragmentation in apoptosis induced by mitochondrial perturbing agents. Apoptosis. 2008·Feb; 13(2):197‑212. <·PMID:·18157742·>
• Townsend KP, Vendrame M, Ehrhart J, Faza B, Zeng J, Town T, Tan J: CD45 isoform RB as a molecular target to oppose lipopolysaccharide‑induced microglial activation in mice. Neurosci·Lett. 2004·May·13; 362(1):26‑30. <·PMID:·15147773·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN