CyFlow CD48 FITC
品番 | AN944761 | ||
---|---|---|---|
抗体名 | Anti-Hu CD48 FITC,MEM-102 | ||
包装単位 | 100 tests | ||
濃度 | - | ||
容量 | 2 ml | ||
関連製品 (アイソタイプコントロール) |
Mouse IgG1 FITC (CD051682) |
||
反応性|交差吸着 | Human | Non-Human Primates | レーザー | Blue |
抗原 | CD48, BLAST-1, BCM1, SLAMF2 | 最大蛍光波長 | 518 nm |
クローン | MEM-102 | 最大励起波長 | 490 to 495 nm |
ホスト | Mouse | 標識/Format | FITC |
アイソタイプ | IgG1 | 研究分野 | Immunophenotyping |
クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu CD48 FITC,MEM-102
特異性
The mouse monoclonal antibody MEM-102 recognizes CD48 antigen, a 40-47 kDa GPI-anchored membrane protein (immunoglobulin supergene family) widely expressed on hematopoietic cells; it is negative on granulocytes, platelets and erythrocytes.
抗原情報
CD48 (Blast-1) belongs to the CD2 subset of the Ig superfamily, which includes CD2, CD2F-10, CD58, CD84, CD150, CD229, CD244 and others. These molecules bind to the same or another members of their family, thus mediate homotypic or heterotypic adhesion. CD48 is a GPI-anchored protein broadly expressed on hematopoietic cells and serves as a high affinity ligand for 2B4 and low affinity ligand for CD2. 2B4-CD48 interaction among NK cells and NK-T cells regulates cell proliferation. Signaling through CD48 results in eosinophil activation and CD48 expression is increased in several infectious diseases.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Angelisová P, Drbal K, Horejsí V, Cerný J: Association of CD10/neutral endopeptidase 24.11 with membrane microdomains rich in glycosylphosphatidylinositol‑anchored proteins and Lyn kinase. Blood. 1999·Feb·15; 93(4):1437‑9. <·PMID:·10075459·>
• Assarsson E, Kambayashi T, Persson CM, Chambers BJ, Ljunggren HG: 2B4/CD48‑mediated regulation of lymphocyte activation and function. J·Immunol. 2005·Aug·15; 175(4):2045‑9. <·PMID:·16081768·>
• Bazil V, Stefanova I, Hilgert I, Kristofova H, Vanek S, Bukovsky A, Horejsi V: Monoclonal antibodies against human leucocyte antigens (III): Antibodies against CD45R, CD6, CD44 and two newly described broadly expressed glycoproteins MEM‑53 and MEM‑102. Folia·Biol·(Praha). 1989; 35(5):289‑97. <·PMID:·2534480·>
• Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J·Clin·Pathol. 1993·Apr; 46(4):334‑6. <·PMID:·7684403·>
• Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single‑molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int·Immunol. 2007·May; 19(5):675‑84. <·PMID:·17446208·>
• Korinek V, Stefanova I, Angelisova P, Hilgert I, Horejsi V: The human leucocyte antigen CD48 (MEM‑102) is closely related to the activation marker Blast‑1. Immunogenetics. 1991; 33(2):108‑12. <·PMID:·1999350·>
• Lee KM, Forman JP, McNerney ME, Stepp S, Kuppireddi S, Guzior D, Latchman YE, Sayegh MH, Yagita H, Park CK, Oh SB, Wülfing C, Schatzle J, Mathew PA, Sharpe AH,Kumar V: Requirement of homotypic NK‑cell interactions through 2B4(CD244)/CD48 in the generation of NK effector functions. Blood. 2006·Apr·15; 107(8):3181‑8. <·PMID:·15905190·>
• Mathew SO, Kumaresan PR, Lee JK, Huynh VT, Mathew PA: Mutational analysis of the human 2B4 (CD244)/CD48 interaction: Lys68 and Glu70 in the V domain of 2B4 are critical for CD48 binding and functional activation of NK cells. J·Immunol. 2005·Jul·15; 175(2):1005‑13. <·PMID:·16002700·>
• Munitz A, Bachelet I, Eliashar R, Khodoun M, Finkelman FD, Rothenberg ME, Levi-Schaffer F: CD48 is an allergen and IL‑3‑induced activation molecule on eosinophils. J·Immunol. 2006·Jul·1; 177(1):77‑83. <·PMID:·16785501·>
• Stulnig TM, Berger M, Sigmund T, Stockinger H, Horejsí V, Waldhäusl W: Signal transduction via glycosyl phosphatidylinositol‑anchored proteins in T cells is inhibited by lowering cellular cholesterol. J·Biol·Chem. 1997·Aug·1; 272(31):19242‑7. <·PMID:·9235917·>
• Knapp W, Dorken B, Gilks W, Rieber EP, Schmidt RE, Stein H, von dem Borne AEGK (Eds): Leucocyte Typing IV. Oxford·University·Press,·Oxford. 1989; 1‑1820. <·NLM·ID:·8914679·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN