CyFlow CD235a FITC
品番 | AN126965 | ||
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抗体名 | Anti-H/R CD235A FITC,JC159 | ||
包装単位 | 100 tests | ||
濃度 | - | ||
容量 | 0.4 ml | ||
関連製品 (アイソタイプコントロール) |
Mouse IgG1 FITC (CD051682) |
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反応性|交差吸着 | Human | Rat | レーザー | Blue |
抗原 | CD235a, Glycophorin A | 最大蛍光波長 | 518 nm |
クローン | JC159 | 最大励起波長 | 490 to 495 nm |
ホスト | Mouse | 標識/Format | FITC |
アイソタイプ | IgG1 | 研究分野 | Immunophenotyping |
クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-H/R CD235A FITC,JC159
特異性
The mouse monoclonal antibody JC159 recognizes an epitope between amino acids 27 and 40 of the extracellular portion of CD235a (glycophorin A), a sialoglycoprotein expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL. The antibody does not react with glycophorin B.
抗原情報
CD235a (GPA; Glycophorin A) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to glycophorin B (GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, an Streptococcus adhesin.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Alijotas-Reig J, Palacio-Garcia C, Llurba E, Vilardell-Tarres M: Cell‑derived microparticles and vascular pregnancy complications: a systematic and comprehensive review. Fertil·Steril. 2013·Feb; 99(2):441‑9. <·PMID:·23122952·>
• Beck Z, Jagodzinski LL, Eller MA, Thelian D, Matyas GR, Kunz AN, Alving CR: Platelets and erythrocyte‑bound platelets bind infectious HIV‑1 in plasma of chronically infected patients. PLoS·One. 2013·Nov·25; 8(11):e81002. <·PMID:·24282562·>
• Maijenburg MW, Kleijer M, Vermeul K, Mul EP, van Alphen FP, van der Schoot CE, Voermans C: The composition of the mesenchymal stromal cell compartment in human bone marrow changes during development and aging. Haematologica. 2012·Feb; 97(2):179‑83. <·PMID:·21993672·>
• Yamauchi T, Takenaka K, Urata S, Shima T, Kikushige Y, Tokuyama T, Iwamoto C, Nishihara M, Iwasaki H, Miyamoto T, Honma N, Nakao M, Matozaki T, Akashi K: Polymorphic Sirpa is the genetic determinant for NOD‑based mouse lines to achieve efficient human cell engraftment. Blood. 2013·Feb·21; 121(8):1316‑25. <·PMID:·23293079·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN