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CyFlow CD62P PE

品番 AM736744
抗体名 Anti-Hu CD62P PE,AK4
包装単位 100 tests
濃度 -
容量 2 ml
関連製品
(アイソタイプコントロール)
Mouse IgG1 PE (BV655324)
反応性|交差吸着 Human | Non-Human Primates レーザー Blue, Green, Yellow
抗原 CD62P, P-selectin 最大蛍光波長 576 nm
クローン AK4 最大励起波長 496 nm, 565 nm
ホスト Mouse 標識/Format PE
アイソタイプ IgG1 研究分野 Immunophenotyping
クローナリティ monoclonal 用途

Flow cytometry

Anti-Hu CD62P PE,AK4

特異性

The mouse monoclonal antibody AK4 recognizes CD62P antigen, a 140 kD single chain type I transmembrane glycoprotein present in secretory α-granules in platelets, in Weibel-Palade bodies in endothelial cells and in megakaryocytes; it is relocated to the plasma membrane upon activation.

抗原情報

CD62P (P-selectin) is an adhesion glycoprotein that is expressed on platelets and endothelial cells upon their activation. Interaction between CD62P and its mucin-like ligand PSGL-1 (P-selectin glycoprotein ligand-1) expressed on the microvilli of most leukocytes supports leukocyte rolling along postkapillary venules at the earliest time of inflammation. Both CD62P and PSGL-1 are extended glycoproteins that form homodimers. CD62P dimerization is probably mediated through interactions of the transmembrane domains and stabilizes leukocyte tethering and rolling, probably by increasing rebinding within a bond cluster.

利用方法

The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Dunlop LC, Skinner MP, Bendall LJ, Favaloro EJ, Castaldi PA, Gorman JJ, Gamble JR, Vadas MA, Berndt MC: Characterization of GMP‑140 (P‑selectin) as a circulating plasma protein. J·Exp·Med. 1992·Apr·1; 175(4):1147‑50. <·PMID:·1372646·>
• Holme PA, Müller F, Solum NO, Brosstad F, Frøland SS, Aukrust P: Enhanced activation of platelets with abnormal release of RANTES in human immunodeficiency virus type 1 infection. FASEB·J. 1998·Jan; 12(1):79‑89. <·PMID:·9438413·>
• Kowalska MA, Ratajczak J, Hoxie J, Brass LF, Gewirtz A, Poncz M, Ratajczak MZ: Megakaryocyte precursors, megakaryocytes and platelets express the HIV co‑receptor CXCR4 on their surface: determination of response to stromal‑derived factor‑1 by megakaryocytes and platelets. Br·J·Haematol. 1999·Feb; 104(2):220‑9. <·PMID:·10050701·>
• Ludwig RJ, Schultz JE, Boehncke WH, Podda M, Tandi C, Krombach F, Baatz H, Kaufmann R, von Andrian UH, Zollner TM: Activated, not resting, platelets increase leukocyte rolling in murine skin utilizing a distinct set of adhesion molecules. J·Invest·Dermatol. 2004·Mar; 122(3):830‑6. <·PMID:·15086572·>

Available Safety Data Sheets

CyFlow™ monoclonal antibodies SDS CA EN

Available Technical Data Sheets

CyFlow™ CD62P PE (AK4) AM736744 TDS (US)