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CyFlow CD8 Biotin

品番 AM060913
抗体名 Anti-Hu CD8 BIOTIN,MEM-31
包装単位 0.1 mg
濃度 1 mg/ml
容量 0.1 ml
関連製品
(アイソタイプコントロール)
Mouse IgG2a Biotin (BH298720)
反応性|交差吸着 Human レーザー
抗原 CD8 最大蛍光波長 -
クローン MEM-31 最大励起波長 -
ホスト Mouse 標識/Format Biotin
アイソタイプ IgG2a 研究分野 Immunophenotyping
クローナリティ monoclonal 用途

Flow cytometry

Anti-Hu CD8 BIOTIN,MEM-31

特異性

The mouse monoclonal antibody MEM-31 recognizes a conformationally-dependent epitope of CD8 antigen, a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. CD8 is a disulfide-linked dimer and exists as a CD8 α/α homodimer or CD8 α/β heterodimer (each monomer approx. 32-34 kDa).
The antibody does not react with formaldehyde-fixed cells; negative in Western Blotting application.

抗原情報

The CD8 T cell coreceptor (monomer approx. 32-34 kDa) is expressed as α/β heterodimer on majority of MHC I-restricted conventional T cells and thymocytes and as α/α homodimer on subsets of memory T cells, intraepithelial lymphocytes, NK cells and dendritic cells. Regulation of CD8 β level on T cell surface seems to be an important mechanism to control their effector function. Assembly of CD8 α-β but not α-α dimers is connected with formation or localization to the lipid rafts. Recruiting triggered TCR complexes to these membrane microdomains as well as affinity of TCR to MHC I is modulated by CD8, thereby affecting the functional diversity of the TCR signaling.

利用方法

The reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working usage is 0.4 µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Brdicková N, Brdicka T, Angelisová P, Horváth O, Spicka J, Hilgert I, Paces J, Simeoni L, Kliche S, Merten C, Schraven B, Horejsí V: LIME: a new membrane Raft‑associated adaptor protein involved in CD4 and CD8 coreceptor signaling. J·Exp·Med. 2003·Nov·17; 198(10):1453‑62. <·PMID:·14610046·>
• Devine L, Thakral D, Nag S, Dobbins J, Hodsdon ME, Kavathas PB: Mapping the binding site on CD8 beta for MHC class I reveals mutants with enhanced binding. J·Immunol. 2006·Sep·15; 177(6):3930‑8. <·PMID:·16951356·>
• Drbal K, Moertelmaier M, Holzhauser C, Muhammad A, Fuertbauer E, Howorka S, Hinterberger M, Stockinger H, Schütz GJ: Single‑molecule microscopy reveals heterogeneous dynamics of lipid raft components upon TCR engagement. Int·Immunol. 2007·May; 19(5):675‑84. <·PMID:·17446208·>
• Estefanía E, Flores R, Gómez-Lozano N, Aguilar H, López-Botet M, Vilches C: Human KIR2DL5 is an inhibitory receptor expressed on the surface of NK and T lymphocyte subsets. J·Immunol. 2007·Apr·1; 178(7):4402‑10. <·PMID:·17371997·>
• Horejsí V, Angelisová P, Bazil V, Kristofová H, Stoyanov S, Stefanová I, Hausner P, Vosecký M, Hilgert I: Monoclonal antibodies against human leucocyte antigens (II); Antibodies against CD45 (T200), CD3 (T3), CD43, CD10 (CALLA), transferrin receptor (T9), a novel broadly expressed 18‑kDa antigen (MEM‑43) and a novel antigen of restricted expression (MEM‑74). Folia·Biol·(Praha). 1988; 34(1):23‑34. <·PMID:·2968928·>
• Horejsi V, Hilgert I, Kristofova H, Bazil V, Bukovsky A, Kulhankova J: Monoclonal antibodies against human leucocyte antigens (I); Antibodies against beta‑2‑microglobulin, immunoglobulin kappa light chains, HLA‑DR‑like antigens, T8 antigen, T1 antigen, a monocyte antigen, and a pan‑leucocyte antigen. Folia·Biol·(Praha). 1986; 32(1):12‑25. <·PMID:·2422063·>
• Linnebacher M, Wienck A, Boeck I, Klar E: Identification of an MSI‑H tumor‑specific cytotoxic T cell epitope generated by the ‑1 frame of U79260(FTO). J·Biomed·Biotechnol. 2010; 2010:841451. <·PMID:·20339516·>
• Pang DJ, Hayday AC, Bijlmakers MJ: CD8 Raft localization is induced by its assembly into CD8alpha beta heterodimers, Not CD8alpha alpha homodimers. J·Biol·Chem. 2007·May·4; 282(18):13884‑94. <·PMID:·17341584·>
• van den Berg HA, Wooldridge L, Laugel B, Sewell AK: Coreceptor CD8‑driven modulation of T cell antigen receptor specificity. J·Theor·Biol. 2007·Nov·21; 249(2):395‑408. <·PMID:·17869274·>
• McMichael AJ, Beverley PCL, Cobbold S, et al (Eds): Leucocyte Typing III, White Cell Differentiation Antigens. Oxford·University·Press,·Oxford. 1987; 1‑1050. <·NLM·ID:·8913266·>

Available Safety Data Sheets

CyFlow™ monoclonal antibodies SDS CA EN

Available Technical Data Sheets

CyFlow™ CD8 Biotin (MEM-31) AM060913 TDS (US)