CyFlow CD50 Biotin
| 品番 | AL442218 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu CD50 BIOTIN,MEM-171 | ||
| 包装単位 | 0.1 mg | ||
| 濃度 | 1 mg/ml | ||
| 容量 | 0.1 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG1 Biotin (AQ807476) |
||
| 反応性|交差吸着 | Human | レーザー | |
| 抗原 | CD50, ICAM-3 | 最大蛍光波長 | - |
| クローン | MEM-171 | 最大励起波長 | - |
| ホスト | Mouse | 標識/Format | Biotin |
| アイソタイプ | IgG1 | 研究分野 | Immunophenotyping |
| クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu CD50 BIOTIN,MEM-171
特異性
The mouse monoclonal antibody MEM-171 recognizes an epitope in the D2 domain of CD50 antigen, a 120-130 kDa type I membrane protein (immunoglobulin supergene family) expressed on leukocytes, endothelial cells and Langerhans cells; it is negative on platelets and erythrocytes.
抗原情報
CD50 ( ICAM-3; intracellular adhesion molecule 3) is a transmembrane glycoprotein expressed by leukocytes, that serves as a counter-receptor for the lymphocyte function-associated antigen (LFA)-1 integrin. Besides functioning as an adhesive molecule that mediates e.g. the contact between T cells and antigen presenting cells, ICAM-3 regulates affinity of LFA-1 for ICAM-1 and induces T cell activation and proliferation. ICAM-3 plays an essential role in the initiation of the immune response both on T cells and antigen presenting cells and interacts also with CD209 (DC-SIGN; dendritic cell-specific ICAM-3-grabbing nonintegrin), a C-type lectin of dendritic cells and macrophages; this process is involved in dialogue between dendritic cells and granulocytes.
利用方法
The reagent is designed for indirect immunofluorescence analysis by Flow Cytometry. Suggested working usage is 2 µg/ml. Indicated dilution is recommended starting point for use of this product, but working concentrations should be validated by the investigator.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Arroyo AG, Campanero MR, Sánchez-Mateos P, Zapata JM, Ursa MA, del Pozo MA, Sánchez-Madrid F: Induction of tyrosine phosphorylation during ICAM‑3 and LFA‑1‑mediated intercellular adhesion, and its regulation by the CD45 tyrosine phosphatase. J·Cell·Biol. 1994·Sep; 126(5):1277‑86. <·PMID:·7520448·>
• Bogoevska V, Nollau P, Lucka L, Grunow D, Klampe B, Uotila LM, Samsen A, Gahmberg CG, Wagener C: DC‑SIGN binds ICAM‑3 isolated from peripheral human leukocytes through Lewis x residues. Glycobiology. 2007·Mar; 17(3):324‑33. <·PMID:·17145745·>
• Campanero MR, del Pozo MA, Arroyo AG, Sánchez-Mateos P, Hernández-Caselles T, Craig A, Pulido R, Sánchez-Madrid F: ICAM‑3 interacts with LFA‑1 and regulates the LFA‑1/ICAM‑1 cell adhesion pathway. J·Cell·Biol. 1993·Nov; 123(4):1007‑16. <·PMID:·7901223·>
• Cermák L, Símová S, Pintzas A, Horejsí V, Andera L: Molecular mechanisms involved in CD43‑mediated apoptosis of TF‑1 cells: Roles of transcription Daxx expression, and adhesion molecules. J·Biol·Chem. 2002·Mar·8; 277(10):7955‑61. <·PMID:·11773067·>
• Filatov AV, Krotov GI, Zgoda VG, Volkov Y: Fluorescent immunoprecipitation analysis of cell surface proteins: a methodology compatible with mass‑spectrometry. J·Immunol·Methods. 2007·Jan·30; 319(1‑2):21‑33. <·PMID:·17098248·>
• Hernandez-Caselles T, Rubio G, Campanero MR, del Pozo MA, Muro M, Sanchez-Madrid F, Aparicio P: ICAM‑3, the third LFA‑1 counterreceptor, is a co‑stimulatory molecule for both resting and activated T lymphocytes. Eur·J·Immunol. 1993·Nov; 23(11):2799‑806. <·PMID:·8223855·>
• Linnebacher M, Wienck A, Boeck I, Klar E: Identification of an MSI‑H tumor‑specific cytotoxic T cell epitope generated by the ‑1 frame of U79260(FTO). J·Biomed·Biotechnol. 2010; 2010:841451. <·PMID:·20339516·>
• Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland·Publishing·Inc,·New·York. 1997; 1‑1342. <·NLM·ID:·9712219·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN