CyFlow CD163 PE-Cy7
品番 | AJ485939 | ||
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抗体名 | Anti-Hu CD163 PE-CY7,GHI/61 | ||
包装単位 | 100 tests | ||
濃度 | - | ||
容量 | 0.4 ml | ||
関連製品 (アイソタイプコントロール) |
Mouse IgG1 PE-Cy7 (CF148611) |
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反応性|交差吸着 | Human | レーザー | Blue, Green, Yellow |
抗原 | CD163, GH1/61, D11, RM3/1, M130 | 最大蛍光波長 | 785 nm |
クローン | GHI/61 | 最大励起波長 | 496 nm, 565 nm |
ホスト | Mouse | 標識/Format | PE-Cy7 |
アイソタイプ | IgG1 | 研究分野 | Immunophenotyping |
クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu CD163 PE-CY7,GHI/61
特異性
The mouse monoclonal antibody GHI/61 recognizes CD163 antigen, a 130 kDa high affinity scavenger receptor expressed mainly on monocytes and macrophages, which binds hemoglobin-haptoglobin complex.
抗原情報
CD163 (M130) is a member of the scavenger receptor family, accounting for the clearance of hemoglobin-haptoglobin complexes during limited hemolysis, which protects the body, in particular the kidneys, against heme-mediated oxidative damages. It does not have measurable affinity for noncomplexed hemoglobin or haptoglobin. Immunomodulatory role of CD163 has been postulated. CD163 is expressed by cells of the monocyte-macrophage lineage and its extracellular part also circulates in plasma as a soluble protein, especially during sepsis and other conditions affecting macrophage activity, when its level may raise manyfold.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Bover LC, Cardó-Vila M, Kuniyasu A, Sun J, Rangel R, Takeya M, Aggarwal BB, Arap W, Pasqualini R: A previously unrecognized protein‑protein interaction between TWEAK and CD163: potential biological implications. J·Immunol. 2007·Jun·15; 178(12):8183‑94. <·PMID:·17548657·>
• Kim WK, Alvarez X, Fisher J, Bronfin B, Westmoreland S, McLaurin J, Williams K: CD163 identifies perivascular macrophages in normal and viral encephalitic brains and potential precursors to perivascular macrophages in blood. Am·J·Pathol. 2006·Mar; 168(3):822‑34. <·PMID:·16507898·>
• Kusi KA, Gyan BA, Goka BQ, Dodoo D, Obeng-Adjei G, Troye-Blomberg M, Akanmori BD, Adjimani JP: Levels of soluble CD163 and severity of malaria in children in Ghana. Clin·Vaccine·Immunol. 2008·Sep; 15(9):1456‑60. <·PMID:·18632918·>
• Law SK, Micklem KJ, Shaw JM, Zhang XP, Dong Y, Willis AC, Mason DY: A new macrophage differentiation antigen which is a member of the scavenger receptor superfamily. Eur·J·Immunol. 1993·Sep; 23(9):2320‑5. <·PMID:·8370408·>
• Madsen M, Møller HJ, Nielsen MJ, Jacobsen C, Graversen JH, van den Berg T, Moestrup SK: Molecular characterization of the haptoglobin‑hemoglobin receptor CD163: Ligand binding properties of the scavenger receptor cysteine‑rich domain region. J·Biol·Chem. 2004·Dec·3; 279(49):51561‑7. <·PMID:·15448162·>
• Møller HJ, Peterslund NA, Graversen JH, Moestrup SK: Identification of the hemoglobin scavenger receptor/CD163 as a natural soluble protein in plasma. Blood. 2002·Jan·1; 99(1):378‑80. <·PMID:·11756196·>
• Moniuszko M, Kowal K, Rusak M, Pietruczuk M, Dabrowska M, Bodzenta-Lukaszyk A: Monocyte CD163 and CD36 expression in human whole blood and isolated mononuclear cell samples: influence of different anticoagulants. Clin·Vaccine·Immunol. 2006·Jun; 13(6):704‑7. <·PMID:·16760331·>
• Philippidis P, Mason JC, Evans BJ, Nadra I, Taylor KM, Haskard DO, Landis RC: Hemoglobin scavenger receptor CD163 mediates interleukin‑10 release and heme oxygenase‑1 synthesis: antiinflammatory monocyte‑macrophage responses in vitro, in resolving skin blisters in vivo, and after cardiopulmonary bypass surgery. Circ·Res. 2004·Jan·9; 94(1):119‑26. <·PMID:·14656926·>
• Pulford K, Micklem K, McCarthy S, Cordell J, Jones M, Mason DY: A monocyte/macrophage antigen recognized by the four antibodies GHI/61, Ber‑MAC3, Ki‑M8 and SM4. Immunology. 1992·Apr; 75(4):588‑95. <·PMID:·1592433·>
• Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland·Publishing·Inc,·New·York. 1997; 1‑1342. <·NLM·ID:·9712219·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN