特異性

The mouse monoclonal antibody mN1A recognizes intracellular domain of Notch 1 protein, mainly its activated form. The unprocessed Notch 1 protein is recognized with lower affinity.

抗原情報

Notch 1 is a 270-300 kDa transmembrane heterodimeric protein with multiple extracellular growth factor-like repeats, and with an intracellular domain consisting of multiple different domain types. It serves as a receptor for membrane ligands, such as Delta 1, Jagged1 (CD339), and Jagged2, and regulates cell fate decisions. Upon ligand binding the transmembrane form of Notch 1 is repeatedly cleaved to provide approximately 120 kDa Notch intracellular fragment (NICD), which translocates to the nucleus and acts as a part of transcriptional complexes that alter differentiation, proliferation, and apoptosis. The highest level of Notch 1 expression is in brain, lung and thymus.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Espinosa L, Santos S, Inglés-Esteve J, Muñoz-Canoves P, Bigas A: p65‑NFkappaB synergizes with Notch to activate transcription by triggering cytoplasmic translocation of the nuclear receptor corepressor N‑CoR. J·Cell·Sci. 2002·Mar·15; 115(6):1295‑303. <·PMID:·11884528·>
• Huppert SS, Le A, Schroeter EH, Mumm JS, Saxena MT, Milner LA, Kopan R: Embryonic lethality in mice homozygous for a processing‑deficient allele of Notch1. Nature. 2000·Jun·22; 405(6789):966‑70. <·PMID:·10879540·>
• Kefas B, Comeau L, Floyd DH, Seleverstov O, Godlewski J, Schmittgen T, Jiang J, diPierro CG, Li Y, Chiocca EA, Lee J, Fine H, Abounader R, Lawler S, Purow B: The neuronal microRNA miR‑326 acts in a feedback loop with notch and has therapeutic potential against brain tumors. J·Neurosci. 2009·Dec·2; 29(48):15161‑8. <·PMID:·19955368·>
• Khwaja SS, Liu H, Tong C, Jin F, Pear WS, van Deursen J, Bram RJ: HIV‑1 Rev‑binding protein accelerates cellular uptake of iron to drive Notch‑induced T cell leukemogenesis in mice. J·Clin·Invest. 2010·Jul·1; 120(7):2537‑48. <·PMID:·20516639·>
• Sun H, Li L, Vercherat C, Gulbagci NT, Acharjee S, Li J, Chung TK, Thin TH, Taneja R: Stra13 regulates satellite cell activation by antagonizing Notch signaling. J·Cell·Biol. 2007·May·21; 177(4):647‑57. <·PMID:·17502421·>
• Tagami S, Okochi M, Yanagida K, Ikuta A, Fukumori A, Matsumoto N, Ishizuka-Katsura Y, Nakayama T, Itoh N, Jiang J, Nishitomi K, Kamino K, Morihara T, Hashimoto R, Tanaka T, Kudo T, Chiba S, Takeda M: Regulation of Notch signaling by dynamic changes in the precision of S3 cleavage of Notch‑1. Mol·Cell·Biol. 2008·Jan; 28(1):165‑76. <·PMID:·17967888·>
• Watanabe K, Nagaoka T, Lee JM, Bianco C, Gonzales M, Castro NP, Rangel MC, Sakamoto K, Sun Y, Callahan R, Salomon DS: Enhancement of Notch receptor maturation and signaling sensitivity by Cripto‑1. J·Cell·Biol. 2009·Nov·2; 187(3):343‑53. <·PMID:·19948478·>