CyFlow CD148 APC
| 品番 | AJ142881 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu CD148 APC,MEM-CD148/05 | ||
| 包装単位 | 100 tests | ||
| 濃度 | - | ||
| 容量 | 1 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG2b APC (BS969970) |
||
| 反応性|交差吸着 | Human | レーザー | Red |
| 抗原 | CD148, DEP-1, HPTP-a, SCC1 | 最大蛍光波長 | 660 nm |
| クローン | MEM-CD148/05 | 最大励起波長 | 650 nm |
| ホスト | Mouse | 標識/Format | APC |
| アイソタイプ | IgG2b | 研究分野 | Immunophenotyping | Phosphorylation |
| クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu CD148 APC,MEM-CD148/05
特異性
The mouse monoclonal antibody MEM-CD148/05 recognizes CD148 antigen, a highly glycosylated up to 250 kDa receptor-like protein tyrosin phosphatase expressed mainly in lymphocytes, myeloid cells and epithelial cells.
抗原情報
CD148 (HPTP-eta, DEP-1) is a transmembrane protein tyrosin phosphatase containing eight fibronectin type III extracellular domains. This protein is known to inhibit transduction of mitogenic signals in non-hematopoietic cells (fibroblasts, epithelial cells), and signal transduction downstream of T cell receptor, however, it also augments immunoreceptor signaling in B cells and macrophages via dephosphorylating C-terminal tyrosine of Src-family tyrosine kinases. CD148 expression increases after in vitro activation of peripheral blood leukocytes. It can be also used as marker of the most mature human thymocytes, and leukemic cells corresponding to this stadium of thymocyte differentiation. In contrast, in mice the CD148 expression sharply drops through the double positive stage to the single positive thymocytes.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Stepanek O, Kalina T, Draber P, Skopcova T, Svojgr K, Angelisova P, Horejsi V, Weiss A, Brdicka T: Regulation of Src family kinases involved in T cell receptor signaling by protein‑tyrosine phosphatase CD148. J·Biol·Chem. 2011·Jun·24; 286(25):22101‑12. <·PMID:·21543337·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN