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CyFlow CD34 PE-Cy5

品番 AG891077
抗体名 Anti-Hu CD34 PE-CY5,581
包装単位 100 tests
濃度 -
容量 0.4 ml
関連製品
(アイソタイプコントロール)
Mouse IgG1 PE-Cy5 (AJ384786)
反応性|交差吸着 Human | Non-Human Primates レーザー Blue, Green, Yellow
抗原 CD34 最大蛍光波長 670 nm
クローン 581 最大励起波長 496 nm, 565 nm
ホスト Mouse 標識/Format PE-Cy5
アイソタイプ IgG1 研究分野 Immunophenotyping
クローナリティ monoclonal 用途

Flow cytometry

Anti-Hu CD34 PE-CY5,581

特異性

The mouse monoclonal antibody 581 recognizes CD34 antigen, a 110-115 kDa monomeric transmembrane phosphoglycoprotein expressed on hematopoietic progenitors cells and on the most pluripotential stem cells; it is gradually lost on progenitor cells. The antibody recognizes the class III CD34 epitope resistant to neuraminidase, chymopapain and glycoprotease.

抗原情報

CD34 (Mucosialin) is a highly glycosylated monomeric 111-115 kDa surface protein, which is present on many stem cell populations. It is a well established stem cell marker, though its expression on human hematopoietic stem cells is reversible. CD34 probably serves as a surface receptor that undergoes receptor-mediated endocytosis and regulates adhesion, differentiation and proliferation of hematopoietic stem cells and other progenitors. CD34 expression is likely to represent a specific state of hematopoietic development that may have altered adhering properties with expanding and differentiating capabilities in both in vitro and in vivo conditions.

利用方法

The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Ando K, Nakamura Y, Chargui J, Matsuzawa H, Tsuji T, Kato S, Hotta T: Extensive generation of human cord blood CD34(+) stem cells from Lin(‑)CD34(‑) cells in a long‑term in vitro system. Exp·Hematol. 2000·Jun; 28(6):690‑9. <·PMID:·10880755·>
• Felschow DM, McVeigh ML, Hoehn GT, Civin CI, Fackler MJ: The adapter protein CrkL associates with CD34. Blood. 2001·Jun·15; 97(12):3768‑75. <·PMID:·11389015·>
• Goardon N, Nikolousis E, Sternberg A, Chu WK, Craddock C, Richardson P, Benson R, Drayson M, Standen G, Vyas P, Freeman S: Reduced CD38 expression on CD34+ cells as a diagnostic test in myelodysplastic syndromes. Haematologica. 2009·Aug; 94(8):1160‑3. <·PMID:·19644143·>
• Iwasaki H, Kawamoto A, Ishikawa M, Oyamada A, Nakamori S, Nishimura H, Sadamoto K, Horii M, Matsumoto T, Murasawa S, Shibata T, Suehiro S, Asahara T: Dose‑dependent contribution of CD34‑positive cell transplantation to concurrent vasculogenesis and cardiomyogenesis for functional regenerative recovery aftr myocardial infarction. Circulation. 2006·Mar·14; 113(10):1311‑25. <·PMID:·16534028·>
• Janowska-Wieczorek A, Marquez LA, Nabholtz JM, Cabuhat ML, Montaño J, Chang H, Rozmus J, Russell JA, Edwards DR, Turner AR: Growth factors and cytokines upregulate gelatinase expression in bone marrow CD34(+) cells and their transmigration through reconstituted basement membrane. Blood. 1999·May·15; 93(10):3379‑90. <·PMID:·10233890·>
• Kato S, Ando K, Nakamura Y, Muguruma Y, Sato T, Yabe H, Yabe M, Hattori K, Yasuda Y, Hotta T: Absence of a CD34‑ hematopoietic precursor population in recipients of CD34+ stem cell transplantation. Bone·Marrow·Transplant. 2001·Sep; 28(6):587‑95. <·PMID:·11607772·>
• Ninos JM, Jefferies LC, Cogle CR, Kerr WG: The thrombopoietin receptor, c‑Mpl, is a selective surface marker for human hematopoietic stem cells. J·Transl·Med. 2006·Feb·16; 4:9. <·PMID:·16480521·>
• Ono F, Sharma BK, Smith CC, Burnett JW, Aurelian L: CD34+ cells in the peripheral blood transport herpes simplex virus DNA fragments to the skin of patients with erythema multiforme (HAEM).. J·Invest·Dermatol. 2005·Jun; 124(6):1215‑24. <·PMID:·15955097·>
• Sanz E, Muñoz-A N, Monserrat J, Van-Den-Rym A, Escoll P, Ranz I, Alvarez-Mon M, de-la-Hera A: Ordering human CD34+CD10‑CD19+ pre/pro‑B‑cell and CD19‑ common lymphoid progenitor stages in two pro‑B‑cell development pathways. Proc·Natl·Acad·Sci·USA. 2010·Mar·30; 107(13):5925‑30. <·PMID:·20231472·>
• Suárez L, Vidriales MB, García-Laraña J, Sanz G, Moreno MJ, López A, Barrena S, Martínez R, Tormo M, Palomera L, Lavilla E, López-Berges MC, de Santiago M, de Equiza ME, Miguel JF, Orfao A: CD34+ cells from acute myeloid leukemia, myelodysplastic syndromes, andnormal bone marrow display different apoptosis and drug resistance‑associated phenotypes. Clin·Cancer·Res. 2004·Nov·15; 10(22):7599‑606. <·PMID:·15569991·>

Available Safety Data Sheets

CyFlow™ monoclonal antibodies SDS CA EN

Available Technical Data Sheets

CyFlow™ CD34 PE-Cy5 (581) AG891077 TDS (US)