CyFlow CD158d PE
| 品番 | AG800254 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu CD158d PE,mAb#33 | ||
| 包装単位 | 100 tests | ||
| 濃度 | - | ||
| 容量 | 2 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG1 PE (BV655324) |
||
| 反応性|交差吸着 | Human | レーザー | Blue, Green, Yellow |
| 抗原 | CD158d, KIR2DL4 | 最大蛍光波長 | 576 nm |
| クローン | mAb#33 | 最大励起波長 | 496 nm, 565 nm |
| ホスト | Mouse | 標識/Format | PE |
| アイソタイプ | IgG1 | 研究分野 | Immunophenotyping |
| クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu CD158d PE,mAb#33
特異性
The mouse monoclonal antibody mAb#33 (also known as mAb 33 or 33) recognizes extracellular portion of CD158d antigen, a 45 kDa NK cell marker. Cell surface expression and function of CD158d depends on genotype of particular individuals.
抗原情報
CD158d (KIR2DL4) is a KIR family member that shares structural features with both activating and inhibitory receptors and may mediate different functions under different circumstances. It contains cytoplasmic ITIM, suggesting inhibitory function, but also transmembrane domain similar to those of activating KIRs. It has been reported that CD158d serves as an inhibitory receptor for peripheral and uterine NK cells, but its ligation with soluble mAbs (unlike immobilized mAbs) results in activation of IFN-γ secretion. CD158d also binds both membrane form and soluble form of its ligand HLA-G.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Goodridge JP, Witt CS, Christiansen FT, Warren HS: KIR2DL4 (CD158d) genotype influences expression and function in NK cells. J·Immunol. 2003·Aug·15; 171(4):1768‑74. <·PMID:·12902476·>
• LeMaoult J, Zafaranloo K, Le Danff C, Carosella ED: HLA‑G up‑regulates ILT2, ILT3, ILT4, and KIR2DL4 in antigen presenting cells, NK cells, and T cells. FASEB·J. 2005·Apr; 19(6):662‑4. <·PMID:·15670976·>
• Rajagopalan S, Bryceson YT, Kuppusamy SP, Geraghty DE, van der Meer A, Joosten I, Long EO: Activation of NK cells by an endocytosed receptor for soluble HLA‑G. PLoS·Biol. 2006·Jan; 4(1):e9. <·PMID:·16366734·>
• Rajagopalan S, Fu J, Long EO: Cutting edge: induction of IFN‑gamma production but not cytotoxicity by the killer cell Ig‑like receptor KIR2DL4 (CD158d) in resting NK cells. J·Immunol. 2001·Aug·15; 167(4):1877‑81. <·PMID:·11489965·>
• Yan WH, Fan LA: Residues Met76 and Gln79 in HLA‑G alpha1 domain involve in KIR2DL4 recognition. Cell·Res. 2005·Mar; 15(3):176‑82. <·PMID:·15780179·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN