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CyFlow c-Myc Purified

品番 AG408332
抗体名 Anti-Hu C-MyC PURI,9E10
包装単位 0.1 mg
濃度 1 mg/ml
容量 0.1 ml
関連製品
(アイソタイプコントロール)
Mouse IgG1 Purified (BJ561896)
Mouse IgG1 Purified (AS224818)
反応性|交差吸着 Human | Fusion Proteins レーザー
抗原 c-Myc 最大蛍光波長 -
クローン 9E10 最大励起波長 -
ホスト Mouse 標識/Format Purified
アイソタイプ IgG1 研究分野 Epitope tags
クローナリティ monoclonal 用途

Flow cytometry
Immunoprecipitation
Western blot
Immunohistochmistry (paraffin-embedded sections)

Anti-Hu C-MyC PURI,9E10

特異性

The mouse monoclonal antibody 9E10 may be used to detect the c-Myc tag. The c-myc gene (8q24 on human chromosome) is the cellular homologue of the v-myc gene originally isolated from an avian myelocytomatosis virus. The c-Myc protein is a transcription factor (nuclear localization). c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis and cell cycle progression. The phosphorylation of c-Myc has been investigated and previous studies have suggested a functional association between phosphorylation at Thr58/Ser62 by glycogen synthase kinase 3, cyclin-dependent kinase, ERK2 and C-Jun N-terminal Kinase (JNK) in cell proliferation and cell cycle regulation. In normal cells the expression of c-Myc is tightly regulated but in human cancers c-Myc is frequently deregulated. c-Myc is also essential for tumor cell development in vasculogenesis and angiogenesis that distribute blood throughout the cells.

抗原情報

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Baggio R, Burgstaller P, Hale SP, Putney AR, Lane M, Lipovsek D, Wright MC, Roberts RW, Liu R, Szostak JW, Wagner RW: Identification of epitope‑like consensus motifs using mRNA display. J·Mol·Recognit. 2002·May-Jun; 15(3):126‑34. <·PMID:·12203838·>
• Boxer LM, Dang CV: Translocations involving c‑myc and c‑myc function. Oncogene. 2001; 20(40):5595‑5610. <·PMID:·11607812·>
• Cermák L, Símová S, Pintzas A, Horejsí V, Andera L: Molecular mechanisms involved in CD43‑mediated apoptosis of TF‑1 cells: Roles of transcription Daxx expression, and adhesion molecules. J·Biol·Chem. 2002·Mar·8; 277(10):7955‑61. <·PMID:·11773067·>
• Dang CV, Resar LM, Emison E, Kim S, Li Q, Prescott JE, Wonsey D, Zeller K: Function of the c‑Myc oncogenic transcription factor. Exp·Cell·Res. 1999; 253(1):63‑77. <·PMID:·10579912·>
• Evan GI, Lewis GK, Ramsay G, Bishop JM: Isolation of monoclonal antibodies specific for human c‑myc proto‑oncogene product. Mol·Cell·Biol. 1985·Dec; 5(12):3610‑6. <·PMID:·3915782·>
• Fujiwara K, Poikonen K, Aleman L, Valtavaara M, Saksela K, Mayer BJ: A single‑chain antibody/epitope system for functional analysis of protein‑protein interactions. Biochemistry. 2002·Oct·22; 41(42):12729‑38. <·PMID:·12379115·>
• Hilpert K, Hansen G, Wessner H, Kuttner G, Welfle K, Seifert M, Hohne W: Anti‑c‑myc antibody 9E10: epitope key positions and variability characterized using peptide spot synthesis on cellulose. Protein·Eng. 2001·Oct; 14(10):803‑6. <·PMID:·11739900·>
• Hoffman B, Amanullah A, Shafarenko M, Liebermann DA: The proto‑oncogene c‑myc in hematopoietic development and leukemogenesis. Oncogene. 2002; 21(21):3414‑3421. <·PMID:·12032779·>
• Persson H, Hennighausen L, Taub R, DeGrado W, Leder P: Antibodies to human c‑myc oncogene product: evidence of an evolutionarily conserved protein induced during cell proliferation. Science. 1984·Aug·17; 225(4663):687‑93. <·PMID:·6431612·>
• Prendergast GC: Mechanisms of apoptosis by c‑Myc. Oncogene·. 1999; 18(19):2967‑2987. <·PMID:·10378693·>
• Siegel J, Brandner G, Hess RD: Cross‑reactivity of the monoclonal antibody 9E10 with murine c‑MYC. Int·J·Oncol. 1998·Dec; 13(6):1259‑62. <·PMID:·9824641·>
• Spandidos DA, Pintzas A, Kakkanas A, Yiagnisis M, Mahera H, Patra E, Agnantis NJ: Elevated expression of the myc gene in human benign and malignant breast lesions compared to normal tissue. Anticancer·Res·. 1987; 7(6):1299‑304. <·PMID:·3327455·>
• Veracini L, Simon V, Richard V, Schraven B, Horejsi V, Roche S, Benistant C: The Csk‑binding protein PAG regulates PDGF‑induced Src mitogenic signaling via GM1. J·Cell·Biol. 2008·Aug·11; 182(3):603‑14. <·PMID:·18695048·>
• Wang X, Campoli M, Ko E, Luo W, Ferrone S: Enhancement of scFv fragment reactivity with target antigens in binding assays following mixing with anti‑tag monoclonal antibodies. J·Immunol·Methods. 2004·Nov; 294(1‑2):23‑35. <·PMID:·15604013·>

Available Safety Data Sheets

CyFlow™ monoclonal antibodies SDS CA EN

Available Technical Data Sheets

CyFlow™ c-Myc Purified (9E10) AG408332 TDS (US)