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CyFlow CD11c PE

品番 AF853575
抗体名 Anti-Hu CD11C PE,BU15
包装単位 100 tests
濃度 -
容量 2 ml
関連製品
(アイソタイプコントロール)
Mouse IgG1 PE (BV655324)
反応性|交差吸着 Human | Non-Human Primates レーザー Blue, Green, Yellow
抗原 CD11c, p150 最大蛍光波長 576 nm
クローン BU15 最大励起波長 496 nm, 565 nm
ホスト Mouse 標識/Format PE
アイソタイプ IgG1 研究分野 Immunophenotyping
クローナリティ monoclonal 用途

Flow cytometry

Anti-Hu CD11C PE,BU15

特異性

The mouse monoclonal antibody BU15 reacts with CD11c antigen, a 150 kDa integrin expressed mainly on dendritic cells and tissue macrophages.

抗原情報

CD11c (αX integrin subunit) forms complex with CD18 (β2 integrin subunit) and is expressed mainly on tissue macrophages and dendritic cells. CD11c binds to complement fragment iC3b, fibrinogen, VCAM-1 and ICAM-2 or e.g. CD90. Like other β2 integrins, CD11c/CD18 plays roles in cell migration and phagocytosis. Moreover, interaction of CD11c/CD18 with plasminogen regulates plasmin activities, and interaction with heparin counteracts binding of iC3b.

利用方法

The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Angel CE, Lala A, Chen CJ, Edgar SG, Ostrovsky LL, Dunbar PR: CD14+ antigen‑presenting cells in human dermis are less mature than their CD1a+ counterparts. Int·Immunol. 2007·Nov; 19(11):1271‑9. <·PMID:·17804688·>
• Bullard DC, Hu X, Adams JE, Schoeb TR, Barnum SR: p150/95 (CD11c/CD18 expression is required for the development of experimental autoimmune encephalomyelitis. Am·J·Pathol. 2007·Jun; 170(6):2001‑8. <·PMID:·17525267·>
• Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J·Clin·Pathol. 1993·Apr; 46(4):334‑6. <·PMID:·7684403·>
• Gang J, Choi J, Lee JH, Nham SU: Identification of critical residues for plasminogen binding by the alphaXI‑domain of the beta2 integrin, alphaXbeta2. Mol·Cells. 2007·Oct·31; 24(2):240‑6. <·PMID:·17978577·>
• Hogg N, Takacs L, Palmer DG, Selvendran Y, Allen C: The p150,95 molecule is a marker of human mononuclear phagocytes: comparison with expression of class II molecules. Eur·J·Immunol. 1986·Mar; 16(3):240‑8. <·PMID:·3456894·>
• Myou S, Zhu X, Boetticher E, Qin Y, Myo S, Meliton A, Lambertino A, Munoz NM, Hamann KJ, Leff AR: Regulation of adhesion of AML14.3D10 cells by surface clustering of beta2‑integrin caused by ERK‑independent activation of cPLA2. Immunology. 2002·Sep; 107(1):77‑85. <·PMID:·12225365·>
• Rezzonico R, Imbert V, Chicheportiche R, Dayer JM: Ligation of CD11b and CD11c beta(2) integrins by antibodies or soluble CD23 induces macrophage inflammatory protein 1alpha (MIP‑1alpha) and MIP‑1beta production in primary human monocytes through a pathway dependent on nuclear factor‑kappaB. Blood. 2001·May·15; 97(10):2932‑40. <·PMID:·11342414·>
• Sadhu C, Hendrickson L, Dick KO, Potter TG, Staunton DE: Novel Tools for Functional Analysis of CD11c: Activation‑Specific, Activation‑Independent, and Activating Antibodies. J·Immunoassay·Immunochem. 2008; 29(1):42‑57. <·PMID:·18080879·>
• Sadhu C, Ting HJ, Lipsky B, Hensley K, Garcia-Martinez LF, Simon SI, Staunton DE: CD11c/CD18: novel ligands and a role in delayed‑type hypersensitivity. J·Leukoc·Biol. 2007·Jun; 81(6):1395‑403. <·PMID:·17389580·>
• Trowald-Wigh G, Johannisson A, Håkansson L: Canine neutrophil adhesion proteins and Fc‑receptors in healthy dogs and dogs with adhesion protein deficiency, as studied by flow cytometry. Vet·Immunol·Immunopathol. 1993·Oct; 38(3‑4):297‑310. <·PMID:·8291207·>
• Van der Vieren M, Le Trong H, Wood CL, Moore PF, St John T, Staunton DE, Gallatin WM: A novel leukointegrin, alpha d beta 2, binds preferentially to ICAM‑3. Immunity. 1995·Dec; 3(6):683‑90. <·PMID:·8777714·>
• Vorup-Jensen T, Chi L, Gjelstrup LC, Jensen UB, Jewett CA, Xie C, Shimaoka M, Linhardt RJ, Springer TA: Binding between the integrin alphaXbeta2 (CD11c/CD18 and heparin. J·Biol·Chem. 2007·Oct·19; 282(42):30869‑77. <·PMID:·17699512·>
• McMichael AJ, Beverley PCL, Cobbold S, et al (Eds): Leucocyte Typing III, White Cell Differentiation Antigens. Oxford·University·Press,·Oxford. 1987; 1‑1050. <·NLM·ID:·8913266·>
• Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland·Publishing·Inc,·New·York. 1997; 1‑1342. <·NLM·ID:·9712219·>

Available Safety Data Sheets

CyFlow™ monoclonal antibodies SDS CA EN

Available Technical Data Sheets

CyFlow™ CD11c PE (BU15) AF853575 TDS (US)