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CyFlow CD18 Purified

品番 AD113673
抗体名 Anti-Hu CD18 PURI,MEM-48
包装単位 0.1 mg
濃度 1 mg/ml
容量 0.1 ml
関連製品
(アイソタイプコントロール)
Mouse IgG1 Purified (BJ561896)
Mouse IgG1 Purified (AS224818)
反応性|交差吸着 Human レーザー
抗原 CD18, b2 integrin 最大蛍光波長 -
クローン MEM-48 最大励起波長 -
ホスト Mouse 標識/Format Purified
アイソタイプ IgG1 研究分野 Immunophenotyping
クローナリティ monoclonal 用途

Flow cytometry
Immunoprecipitation
Western blot
Immunohistochemistry
Functional assays

Anti-Hu CD18 PURI,MEM-48

特異性

The mouse monoclonal antibody MEM-48 recognizes an epitope involving residues 534-546 in cysteine-rich repeat 3 of the CD18 antigen. CD18 is a 90-95 kDa type I transmembrane protein expressed on all leukocytes.

抗原情報

CD18 (integrin β2 subunit) forms heterodimers with four types of CD11 molecule to constitute leukocyte (β2) integrins: αLβ2 (CD11a/CD18, LFA-1), αMβ2 (CD11b/CD18, Mac-1, CR3), αXβ2 (CD11c/CD18) and αDβ2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits. These integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1; severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Bazil V, Stefanova I, Hilgert I, Kristofova H, Vanek S, Horejsi V: Monoclonal antibodies against human leucocyte antigens (IV): Antibodies against subunits of the LFA‑1 (CD11a/CD18) leucocyte‑adhesion glycoprotein. Folia·Biol·(Praha). 1990; 36(1):41‑50. <·PMID:·1971601·>
• Dao MA, Arevalo J, Nolta JA: Reversibility of CD34 expression on human hematopoietic stem cells that retain the capacity for secondary reconstitution. Blood. 2003·Jan·1; 101(1):112‑8. <·PMID:·12589631·>
• Gannon GA, Rhind SG, Suzui M, Zamecnik J, Sabiston BH, Shek PN, Shephard RJ: beta‑Endorphin and natural killer cell cytolytic activity during prolonged exercise: Is there a connection?. Am·J·Physiol. 1998·Dec; 275(6‑2):R1725‑34. <·PMID:·9843861·>
• Garnotel R, Rittié L, Poitevin S, Monboisse JC, Nguyen P, Potron G, Maquart FX, Randoux A, Gillery P: Human blood monocytes interact with type I collagen through alpha x beta 2 integrin (CD11c‑CD18, gp150‑95). J·Immunol. 2000·Jun·1; 164(11):5928‑34. <·PMID:·10820275·>
• Kerstan A, Hünig T: Cutting edge: distinct TCR‑ and CD28‑derived signals regulate CD95L, Bcl‑xL, and the survival of primary T cells. J·Immunol. 2004·Feb·1; 172(3):1341‑5. <·PMID:·14734708·>
• Kuttruff S, Koch S, Kelp A, Pawelec G, Rammensee HG, Steinle A: NKp80 defines and stimulates a reactive subset of CD8 T cells. Blood. 2009·Jan·8; 113(2):358‑69. <·PMID:·18922855·>
• Larson RS, Springer TA: Structure and function of leukocyte integrins. Immunol·Rev. 1990·Apr; 114:181‑217. <·PMID:·2196220·>
• Ottonello L, Epstein AL, Dapino P, Barbera P, Morone P, Dallegri F: Monoclonal Lym‑1 antibody‑dependent cytolysis by neutrophils exposed to granulocyte‑macrophage colony‑stimulating factor: intervention of FcgammaRII (CD32), CD11b‑CD18 integrins, and CD66b glycoproteins. Blood. 1999·May·15; 93(10):3505‑11. <·PMID:·10233903·>
• Persson H, Hennighausen L, Taub R, DeGrado W, Leder P: Antibodies to human c‑myc oncogene product: evidence of an evolutionarily conserved protein induced during cell proliferation. Science. 1984·Aug·17; 225(4663):687‑93. <·PMID:·6431612·>
• Schiff DE, Rae J, Martin TR, Davis BH, Curnutte JT: Increased phagocyte Fc gammaRI expression and improved Fc gamma‑receptor‑mediated phagocytosis after in vivo recombinant human interferon‑gamma treatment of normal human subjects. Blood. 1997·Oct·15; 90(8):3187‑94. <·PMID:·9376602·>
• Shan M, Klasse PJ, Banerjee K, Dey AK, Iyer SP, Dionisio R, Charles D, Campbell-Gardener L, Olson WC, Sanders RW, Moore JP: HIV‑1 gp120 mannoses induce immunosuppressive responses from dendritic cells. PLoS·Pathog. 2007·Nov; 3(11):e169. <·PMID:·17983270·>
• Solovey AN, Gui L, Chang L, Enenstein J, Browne PV, Hebbel RP: Identification and functional assessment of endothelial P1H12. J·Lab·Clin·Med. 2001·Nov; 138(5):322‑31. <·PMID:·11709656·>
• Stefanova I, Horejsi V: Association of the CD59 and CD55 cell surface glycoproteins with other membrane molecules. J·Immunol. 1991·Sep·1; 147(5):1587‑92. <·PMID:·1715364·>

Available Safety Data Sheets

CyFlow™ monoclonal antibodies SDS CA EN

Available Technical Data Sheets

CyFlow™ CD18 Purified (MEM-48) AD113673 TDS (US)