CyFlow CD7 PerCP-Cy5.5
品番 | AC404264 | ||
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抗体名 | Anti-Hu CD7 PERCP-CY5.5,MEM-186 | ||
包装単位 | 100 tests | ||
濃度 | - | ||
容量 | 0.4 ml | ||
関連製品 (アイソタイプコントロール) |
Mouse IgG1 PerCP-Cy5.5 (CZ886982) |
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反応性|交差吸着 | Human | レーザー | Blue, Green |
抗原 | CD7, gp40 | 最大蛍光波長 | 695 nm |
クローン | MEM-186 | 最大励起波長 | 482 nm |
ホスト | Mouse | 標識/Format | PerCP-Cy5.5 |
アイソタイプ | IgG1 | 研究分野 | Immunophenotyping |
クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu CD7 PERCP-CY5.5,MEM-186
特異性
The mouse monoclonal antibody MEM-186 recognizes CD7 antigen, a 40 kD type I transmembrane glycoprotein expressed on peripheral blood T lymphocytes, NK-cells, hematopoietic progenitors, monocytes (weakly) and also on acute lymphocytic leukemia.
抗原情報
CD7 (gp40) is a member of the immunoglobulin superfamily found on T cells, NK cells, thymocytes, hematopoietic progenitors, and monocytes (weakly). CD7 is also expressed on acute lymphocytic leukemia (ALL). CD7 crosslinking induces a calcium flux in T lymphocytes, presumably as a result of cytoplasmic domain association with PI3-kinase. CD7 co-stimulation can induce cytokine secretion and modulate cellular adhesion. A ligand of CD7, epithelial cell secreted protein K12, is produced in thymus to regulate thymocyte signaling and cytokine release. In lung microvascular endothelial cells CD7 serves as an IgM Fc receptor. Expression of CD7 is an important marker used in leukemia diagnostics.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 4 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Alaibac M, Pigozzi B, Belloni-Fortina A, Michelotto A, Saponeri A, Peserico A: CD7 expression in reactive and malignant human skin T‑lymphocytes. Anticancer·Res. 2003·May-Jun; 23(3B):2707‑10. <·PMID:·12894562·>
• Chang H, Yeung J, Brandwein J, Yi QL: CD7 expression predicts poor disease free survival and post‑remission survival in patients with acute myeloid leukemia and normal karyotype. Leuk·Res. 2007·Feb; 31(2):157‑62. <·PMID:·16837044·>
• Lam GK, Liao HX, Xue Y, Alam SM, Scearce RM, Kaufman RE, Sempowski GD, Haynes BF: Expression of the CD7 ligand K‑12 in human thymic epithelial cells: regulationby IFN‑gamma. J·Clin·Immunol. 2005·Jan; 25(1):41‑9. <·PMID:·15742156·>
• Nishimura M, Takanashi M, Okazaki H, Satake M, Nakajima K: Role of CD7 expressed in lung microvascular endothelial cells as Fc receptor for immunoglobulin M. Endothelium. 2006·Jul-Aug; 13(4):287‑92. <·PMID:·16990185·>
• Kishimoto T, Goyert S, Kikutani H, Mason D, Miyasaka M, Moretta L, Ohno T, Okumura K, Shaw S, Springer TA, Sugamura K, Sugawara H, von dem Borne AEGK, Zola H (Eds): Leucocyte Typing VI. Garland·Publishing·Inc,·New·York. 1997; 1‑1342. <·NLM·ID:·9712219·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN