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CyFlow CD205 PE

品番 AB042675
抗体名 Anti-Hu CD205 PE,HD30
包装単位 100 tests
濃度 -
容量 1 ml
関連製品
(アイソタイプコントロール)
Mouse IgG1 PE (BV655324)
反応性|交差吸着 Human レーザー Blue, Green, Yellow
抗原 CD205, DEC205 最大蛍光波長 576 nm
クローン HD30 最大励起波長 496 nm, 565 nm
ホスト Mouse 標識/Format PE
アイソタイプ IgG1 研究分野 Immunophenotyping
クローナリティ monoclonal 用途

Flow cytometry

Anti-Hu CD205 PE,HD30

特異性

The mouse monoclonal antibody HD30 recognizes CD205 antigen, a 200 kDa C-type lectin transmembrane protein of the MMR (macrophage mannose receptor) family, expressed at high levels on dendritic cells and thymic epithelial cells, and at low levels on lymphocytes, NK cells and monocytes.

抗原情報

CD205 (DEC-205) is an endocytic receptor of macrophage mannose receptor family. This 205 kDa C-type lectin transmembrane protein mediates adsorptive uptake and its intracellular domain contains coated pit localization sequence and distal acidic motif, which is required for recycling beyond early endosomes through deeper MHC II+ late endosomes and lysosomes. This unique pathway of receptor-mediated uptake proves to be necessary for presentation of antigenic peptides at low doses of ligand. CD205 is responsible for uptake and processing of captured antigens for dendritic cells.

利用方法

The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 10 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (1 ml) is sufficient for 100 tests.

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Guo M, Gong S, Maric S, Misulovin Z, Pack M, Mahnke K, Nussenzweig MC, Steinman RM: A monoclonal antibody to the DEC‑205 endocytosis receptor on human dendritic cells. Hum·Immunol. 2000·Aug; 61(8):729‑38. <·PMID:·10980384·>
• Gurer C, Strowig T, Brilot F, Pack M, Trumpfheller C, Arrey F, Park CG, Steinman RM, Münz C: Targeting the nuclear antigen 1 of Epstein‑Barr virus to the human endocytic receptor DEC‑205 stimulates protective T‑cell responses. Blood. 2008·Aug·15; 112(4):1231‑9. <·PMID:·18519810·>
• Mahnke K, Guo M, Lee S, Sepulveda H, Swain SL, Nussenzweig M, Steinman RM: The dendritic cell receptor for endocytosis, DEC‑205, can recycle and enhance antigen presentation via major histocompatibility complex class II‑positive lysosomal compartments. J·Cell·Biol. 2000·Oct·30; 151(3):673‑84. <·PMID:·11062267·>
• Park CG, Rodriguez A, Ueta H, Lee H, Pack M, Matsuno K, Steinman RM: Generation of anti‑human DEC205/CD205 monoclonal antibodies that recognize epitopes conserved in different mammals. J·immunol·Methods. 2012·Mar·30; 377(1‑2):15‑22. <·PMID:·22273672·>

Available Safety Data Sheets

CyFlow™ monoclonal antibodies SDS CA EN

Available Technical Data Sheets

CyFlow™ CD205 PE (HD30) AB042675 TDS (US)