CyFlow CD72 PE
| 品番 | AA664263 | ||
|---|---|---|---|
| 抗体名 | Anti-Hu CD72 PE,3F3 | ||
| 包装単位 | 100 tests | ||
| 濃度 | - | ||
| 容量 | 2 ml | ||
| 関連製品 (アイソタイプコントロール) |
Mouse IgG2b PE (CP922237) |
||
| 反応性|交差吸着 | Human | レーザー | Blue, Green, Yellow |
| 抗原 | CD72, Lyb-2, LYB2 | 最大蛍光波長 | 576 nm |
| クローン | 3F3 | 最大励起波長 | 496 nm, 565 nm |
| ホスト | Mouse | 標識/Format | PE |
| アイソタイプ | IgG2b | 研究分野 | Immunophenotyping |
| クローナリティ | monoclonal | 用途 | Flow cytometry |
Anti-Hu CD72 PE,3F3
特異性
The mouse monoclonal antibody 3F3 recognizes CD72 antigen, a 39-43 kDa type II membrane glycoprotein (C-type lectin family). CD72 is a pan-B cell marker expressed throughout the B lymphocytes diferentiation with the exception of plasma cells; it is also present on follicular dendritic cells.
抗原情報
CD72 is a transmembrane glycoprotein expressed as a homodimer especially in B cells, but also in other antigen presenting cells such as dendritic cells and macrophages. Through one of its immunoreceptor tyrosine-based inhibitory motives (ITIMs), CD72 interacts with tyrosine phosphatase SHP-1, thereby suppressing B cell responsiveness. Binding of CD72 with its ligand CD100 (Sema4D) prevents BCR association and phosphorylation of CD72 and results in dissociation of SHP-1 from CD72, thus enables B cell activation.
利用方法
The reagent is designed for Flow Cytometry analysis of human blood cells. Recommended usage is 20 µl reagent / 100 µl of whole blood or 10^6 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests.
保存方法
Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.
安定性情報
Do not use after expiration date stamped on vial label.
レファレンス
• Dauby N, Kummert C, Lecomte S, Liesnard C, Delforge ML, Donner C, Marchant A: Primary Human Cytomegalovirus Infection Induces the Expansion of Virus‑Specific Activated and Atypical Memory B Cells. J·Infect·Dis. 2014·May·1; <·PMID:·25958387·>
• Doussis IA, Gatter KC, Mason DY: CD68 reactivity of non‑macrophage derived tumours in cytological specimens. J·Clin·Pathol. 1993·Apr; 46(4):334‑6. <·PMID:·7684403·>
• Kish DD, Gorbachev AV, Fairchild RL: CD8+ T cells produce IL‑2, which is required for CD(4+)CD25+ T cell regulation of effector CD8+ T cell development for contact hypersensitivity responses. J·Leukoc·Biol. 2005·Sep; 78(3):725‑35. <·PMID:·16000396·>
• Kumanogoh A, Kikutani H: The CD100‑CD72 interaction: a novel mechanism of immune regulation. Trends·Immunol. 2001·Dec; 22(12):670‑6. <·PMID:·11738997·>
• Kumanogoh A, Shikina T, Watanabe C, Takegahara N, Suzuki K, Yamamoto M, Takamatsu H, Prasad DV, Mizui M, Toyofuku T, Tamura M, Watanabe D, Parnes JR, Kikutani H: Requirement for CD100‑CD72 interactions in fine‑tuning of B‑cell antigen receptor signaling and homeostatic maintenance of the B‑cell compartment. Int·Immunol. 2005·Oct; 17(10):1277‑82. <·PMID:·16113236·>
• Kumanogoh A, Watanabe C, Lee I, Wang X, Shi W, Araki H, Hirata H, Iwahori K, Uchida J, Yasui T, Matsumoto M, Yoshida K, Yakura H, Pan C, Parnes JR, Kikutani H: Identification of CD72 as a lymphocyte receptor for the class IV semaphorin CD100: a novel mechanism for regulating B cell signaling. Immunity. 2000·Nov; 13(5):621‑31. <·PMID:·11114375·>
• Mizrahi S, Markel G, Porgador A, Bushkin Y, Mandelboim O: CD100 on NK cells enhance IFNgamma secretion and killing of target cells expressing CD72. PLoS·One. 2007·Sep·5; 2(9):e818. <·PMID:·17786190·>
• Mizrahi S, Markel G, Porgador A, Bushkin Y, Mandelboim O: CD100 on NK cells enhance IFNgamma secretion and killing of target cells expressing CD72. PLoS·One. 2007·Sep·5; 2(9):e818. <·PMID:·17786190·>
Available Safety Data Sheets
CyFlow™ monoclonal antibodies SDS CA EN