特異性

The mouse monoclonal antibody 2D1 recognizes all alternative forms of human CD45 antigen, a 180-220 kDa single chain type I transmembrane protein expressed at high level on all cells of hematopoietic origin, except from erythrocytes and platelets.

抗原情報

CD45 (LCA; leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.

利用方法

保存方法

Avoid prolonged exposure to light. Store in the dark at 2-8°C. Do not freeze.

安定性情報

Do not use after expiration date stamped on vial label.

レファレンス

• Ito A, Ishida T, Yano H, Inagaki A, Suzuki S, Sato F, Takino H, Mori F, Ri M, Kusumoto S, Komatsu H, Iida S, Inagaki H, Ueda R: Defucosylated anti‑CCR4 monoclonal antibody exercises potent ADCC‑mediated antitumor effect in the novel tumor‑bearing humanized NOD/Shi‑scid, IL‑2Rgamma(null) mouse model. Cancer·Immunol·Immunother. 2009·Aug; 58(8):1195‑206. <·PMID:·19048251·>
• Janossy G, Jani IV, Bradley NJ, Bikoue A, Pitfield T, Glencross DK: Affordable CD4(+)‑T‑cell counting by flow cytometry: CD45 gating for volumetric analysis. Clin·Diagn·Lab·Immunol. 2002·Sep; 9(5):1085‑94. <·PMID:·12204964·>
• Mariucci S, Rovati B, Bencardino K, Manzoni M, Danova M: Flow cytometric detection of circulating endothelial cells and endothelial progenitor cells in healthy subjects. Int·J·Lab·Hematol. 2010·Feb; 32(1‑1):e40‑8. <·PMID:·20088999·>
• Pfau JC, Walker E, Card GL: Monoclonal antibodies to CD45 modify LPS‑induced arachidonic acid metabolism in macrophages. Biochim·Biophys·Acta. 2000·Feb·28; 1495(3):212‑22. <·PMID:·10699460·>
• Vicetti Miguel RD, Harvey SA, LaFramboise WA, Reighard SD, Matthews DB, Cherpes TL: Human female genital tract infection by the obligate intracellular bacterium Chlamydia trachomatis elicits robust Type 2 immunity. PLoS·One. 2013; 8(3):e58565. <·PMID:·23555586·>
• Wellhausen SR, Slone SP, Miller JJ: Clone‑specific anti‑CD45 blocking factor in patient plasma. Cytometry·B·Clin·Cytom. 2007·Sep; 72(5):423‑6. <·PMID:·17311353·>